3-Phosphoglyceric Phosphokinase (PGK), CAS No.9001-83-6

CAS: 9001-83-6 Cat. No.: P1441020 EC Number: 232-636-1

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), ≥700 U/mg protein

Accession #

P00558,P07205

Bioactivity

≥700 U/mg protein

Overview

3-Phosphoglycerate Phosphokinase (or 3-Phosphoglycerate Kinase) catalyzes the reversible transfer of a phosphate group from 1,3-diphosphoglycerate to ADP, generating ATP and 3-phosphoglycerate. The activity of 3-phosphoglycerate phosphokinase is essential for glycolysis and gluconeogenesis. Phosphoglycerate Kinase (PGK) plays an important role in the glycolytic pathway by catalyzing one of the two ATP-producing reactions. It converts 1,3-diphosphoglycerate (1,3-BPGA) to 3-phosphoglycerate (3-PGA). In addition, it participates in gluconeogenesis by catalyzing the reverse reaction to generate 1,3-BPGA and ADP.Because it is involved in many processes besides energy metabolism, such as pathogenesis, interaction with nucleic acids, tumorigenesis and progression, cell death, and viral replication, PGK is also referred to as a moonlighting protein.

Aladdin provides recombinant 3-phosphoglycerate phosphokinase with high enzyme activity and excellent stability. 

Instructions for Use:

1.Study the inhibitory mechanisms of low-molecular-weight GTP-binding proteins and glyceraldehyde-3-phosphate dehydrogenase.

2.Determine the reverse activity of purified rabbit skeletal muscle NAD⁺-dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by coupled assay.

3.Quantitate glyceraldehyde-3-phosphate dehydrogenase.

Specifications

Product Name

3-Phosphoglyceric Phosphokinase (PGK), CAS No.9001-83-6

Synonyms

Phosphoglycerate kinase | ATP:3-phospho-D-glycerate 1-phosphotransferase | PGK | 3-PGK | ATP-3-phospho-D-glycerate-1-phosphotransferase | ATP:D-3-phosphoglycerate 1-phosphotransferase | 3-phosphoglycerate kinase | 3-phosphoglycerate phosphokinase | 3-phos

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), ≥700 U/mg protein

Bioactivity

≥700 U/mg protein

Accession #

P00558, P07205

SDS-PAGE

46 kDa

CAS

9001-83-6

Enzyme Commission Number

EC 2.7.2.3

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥700 U/mg protein

Reconstitution

Reconstitute in pure water.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (24 months). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

One unit is defined as the amount of enzyme required to convert 1.0 μmol of 1,3‑diphosphoglycerate to 3‑phosphoglycerate per minute at pH 6.9 and 25 °C.