GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)
Expression System
HEK293
Accession #
P25942
Protein Tag
C-His
Expression system
HEK293
Endotoxin Concentration
<1.0 EU/μg
Bioactivity
Immobilized Recombinant Human CD40 Protein (rp183577) at 1.0 μg/mL can bind Recombinant Human CD40 Ligand Protein (rp169583) with the EC50 of 20.84 ng/mL. Immobilized Recombinant Human CD40 Protein (rp183577) at 1.0 μg/mL can bind CD40 Mouse mAb (Ab094764) with the EC50 of 15.35 ng/mL. Immobilized Recombinant Human CD40 Protein (rp183577) at 1.0 μg/mL can bind Ravagalimab (anti-CD40) (Ab182860) with the EC50 of 11.32 ng/mL. Immobilized Recombinant Human CD40 Protein (rp183577) at 1.0 μg/mL can bind Mitazalimab (anti-CD40) (Ab182809) with the EC50 of 4.37 ng/mL. Immobilized Recombinant Human CD40 Protein (rp183577) at 1.0 μg/mL can bind Giloralimab (anti-CD40) (Ab182875) with the EC50 of 25.5 ng/mL.
Animal Free,Carrier Free,Bioactive,ActiBioPure™,His Tag,PBS Only,≥95%(SDS-PAGE) ActiBioPure™,Animal Free,Bioactive,Carrier Free,His Tag,PBS Only for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Protein Function: CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.
Immobilized Recombinant Human CD40 Protein (rp183577) at 1.0 μg/mL can bind Recombinant Human CD40 Ligand Protein (rp169583) with the EC50 of 20.84 ng/mL. Immobilized Recombinant Human CD40 Protein (rp183577) at 1.0 μg/mL can bind CD40 Mouse mAb (Ab094764) with the EC50 of 15.35 ng/mL. Immobilized Recombinant Human CD40 Protein (rp183577) at 1.0 μg/mL can bind Ravagalimab (anti-CD40) (Ab182860) with the EC50 of 11.32 ng/mL. Immobilized Recombinant Human CD40 Protein (rp183577) at 1.0 μg/mL can bind Mitazalimab (anti-CD40) (Ab182809) with the EC50 of 4.37 ng/mL. Immobilized Recombinant Human CD40 Protein (rp183577) at 1.0 μg/mL can bind Giloralimab (anti-CD40) (Ab182875) with the EC50 of 25.5 ng/mL.
28.7 kDa, under reducing conditions; 27.9 kDa, under non-reducing conditions
Molecule Type
Protein
Storage and Shipping
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C for 1 year. Avoid freeze / thaw cycle.
Images
Recombinant Human CD40/TNFRSF5 Protein (rp183577) - Binding Activity Immobilized Recombinant Human CD40/TNFRSF5 Protein (rp183577) at 1.0 μg/mL can bind Recombinant Human CD40 Ligand Protein (rp169583) with the EC₅₀ of 20.84 ng/mL.
Recombinant Human CD40/TNFRSF5 Protein (rp183577) - ELISA Immobilized Recombinant Human CD40/TNFRSF5 Protein (rp183577) at 1.0 μg/mL can bind CD40 Mouse mAb (Ab094764) with the EC₅₀ of 15.35 ng/mL.
Recombinant Human CD40/TNFRSF5 Protein (rp183577) - ELISA Immobilized Recombinant Human CD40/TNFRSF5 Protein (rp183577) at 1.0 μg/mL can bind Ravagalimab (anti-CD40) (Ab182860) with the EC₅₀ of 11.32 ng/mL.
Recombinant Human CD40/TNFRSF5 Protein (rp183577) - ELISA Immobilized Recombinant Human CD40/TNFRSF5 Protein (rp183577) at 1.0 μg/mL can bind Mitazalimab (anti-CD40) (Ab182809) with the EC₅₀ of 4.37 ng/mL.
Recombinant Human CD40/TNFRSF5 Protein (rp183577) - ELISA Immobilized Recombinant Human CD40/TNFRSF5 Protein (rp183577) at 1.0 μg/mL can bind Giloralimab (anti-CD40) (Ab182875) with the EC₅₀ of 25.5 ng/mL.
Recombinant Human CD40/TNFRSF5 Protein (rp183577) - SDS-PAGE 3 μg/lane of Recombinant Human CD40/TNFRSF5 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 28.7 kDa under reducing conditions and 27.9 kDa under non-reducing conditions.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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