Recombinant MHC Class II Antibody (Biotin) - Primary antibody, specific to MHC Class II

Cat. No.: Ab213990

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

P24J10

Conjugation

Biotin

Overview

Recombinant MHC Class II Antibody, clone P24J10, could be used for Flow Cytometry.

Specifications

Product Name

Recombinant MHC Class II Antibody (Biotin) - Primary antibody, specific to MHC Class II

Synonyms

D6S221E antibody | DMA antibody | DMB antibody | DP beta 1 antibody | DP beta 1 chain antibody | DP(W4) beta chain antibody | DPB 1 antibody | DPB1 antibody | DPB1_HUMAN antibody | DRB antibody | H2Ea antibody | HLA class II histocompatibility antigen ant

Specifications & Purity

Validated, ExactAb™, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides

Host species

Rat

Specificity

MHC Class II

Immunogen

Activated C57BL/6 mouse spleen cells

Positive Control

Flow: C57BL/6 mouse splenocytes

Conjugation

Biotin

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein A purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: Recombinant MHC Class II Antibody (Biotin) (Ab213990)

Recombinant MHC Class II Antibody (Biotin) (Ab213990) - Flow Cytometry
Flow cytometry analysis of MHC Class II expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant MHC Class II Antibody (Biotin) (Ab213990) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Rat IgG (Biotin) (Ab180310).

Flow Cytometry: Recombinant MHC Class II Antibody (Biotin) (Ab213990)

Recombinant MHC Class II Antibody (Biotin) (Ab213990) - Flow Cytometry
Flow cytometry analysis of MHC Class II expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant MHC Class II Antibody (Biotin) (Ab213990) 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

HLA-DPB1 Tbio HLA class II histocompatibility antigen, DP beta 1 chain (0 Activities)

Discover Target: HLA-DPB1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)