SBI-0206965 - Moligand™, ≥98% , Inhibitor of unc-51 like autophagy activating kinase 1, CAS No.1884220-36-3, Inhibitor of unc-51 like autophagy activating kinase 1

CAS: 1884220-36-3 Cat. No.: S413863 Molecular Weight: 489.32 EC Number: 861-948-6 PubChem CID: 92044402

GRADE & PURITY Moligand™, ≥98%

Synonyms

AS-56287 | GTPL11339 | 2-(5-Bromo-2-(3,4,5-trimethoxyphenylamino)pyrimidin-4-yloxy)-N-methylbenzamide | 4-oxocyclohexylcarboxylic acid ethyl ester | HY-16966 | NSC784816 | NSC-784816 | SBI 0206965 | US10266549, Example 171a | US10266549, Example 88 | CCG-

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

Information

SBI-0206965 SBI-0206965 is a highly selective autophagy kinase ULK1 inhibitor with IC50 of 108 nM, about 7-fold selectivity over ULK2. SBI-0206965 inhibits autophagy and enhances apoptosis in human glioblastoma and lung cancer cells.

Targets

ULK1 (Cell-free assay); ULK2 (Cell-free assay) 108 nM; 711 nM

In vitro

In HEK293T transfected with WT or KI Myc-tagged ULK1 and WT Vps34, SBI-0206965 inhibits Ser249 phosphorylation of overexpressed Vps34 and Beclin1 Ser15 phosphorylation. SBI-0206965 suppresses autophagy induced by mTOR inhibition in A549 cells, and prevents ULK1-dependent cell survival in WT MEFs. In addition, SBI-0206965 also synergizes with mTOR inhibition to induce cancer cell death.

Specifications

Synonyms

AS-56287 | GTPL11339 | 2-(5-Bromo-2-(3, 4, 5-trimethoxyphenylamino)pyrimidin-4-yloxy)-N-methylbenzamide | 4-oxocyclohexylcarboxylic acid ethyl ester | HY-16966 | NSC784816 | NSC-784816 | SBI 0206965 | US10266549, Example 171a | US10266549, Example 88 | CCG-

Specifications & Purity

Moligand™, ≥98%

Biochemical and Physiological Mechanisms

SBI-0206965 is a highly selective autophagy kinase ULK1 inhibitor with IC50 of 108 nM, about 7-fold selectivity over ULK2. SBI-0206965 inhibits autophagy and enhances apoptosis in human glioblastoma and lung cancer cells.

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Moligand™

Action Type

INHIBITOR

Mechanism of action

Inhibitor of unc-51 like autophagy activating kinase 1

Purity

≥98%

Product Properties

• ALogP: 4.128
• HBD Count: 2
• Rotatable Bond: 8

Names and Identifiers

• Canonical Smiles: CNC(=O)C1=CC=CC=C1OC2=NC(=NC=C2Br)NC3=CC(=C(C(=C3)OC)OC)OC
• IUPAC Name: 2-[5-bromo-2-(3,4,5-trimethoxyanilino)pyrimidin-4-yl]oxy-N-methylbenzamide
• InChIKey: NEXGBSJERNQRSV-UHFFFAOYSA-N
• INCHI: 1S/C21H21BrN4O5/c1-23-19(27)13-7-5-6-8-15(13)31-20-14(22)11-24-21(26-20)25-12-9-16(28-2)18(30-4)17(10-12)29-3/h5-11H,1-4H3,(H,23,27)(H,24,25,26)
• Isomeric SMILES: CNC(=O)C1=CC=CC=C1OC2=NC(=NC=C2Br)NC3=CC(=C(C(=C3)OC)OC)OC
• PubChem CID: 92044402
• Molecular Weight: 489.32

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic oxygen compounds
    • Class Organooxygen compounds
      • Subclass Ethers

• Kingdom: Organic compounds
• Superclass: Organic oxygen compounds
• Class: Organooxygen compounds
• Subclass: Ethers
• Intermediate Tree Nodes: Not available
• Direct Parent: Diarylethers
• Alternative Parents: Benzamides Methoxyanilines Anisoles Phenoxy compounds Benzoyl derivatives Methoxybenzenes Alkyl aryl ethers Aminopyrimidines and derivatives Halopyrimidines Aryl bromides Heteroaromatic compounds Secondary carboxylic acid amides Azacyclic compounds Organic oxides Organobromides Amines Hydrocarbon derivatives
• Molecular Framework: Aromatic heteromonocyclic compounds
• Substituents: Diaryl ether - Benzamide - Benzoic acid or derivatives - Methoxyaniline - Phenoxy compound - Anisole - Methoxybenzene - Aniline or substituted anilines - Benzoyl - Phenol ether - Alkyl aryl ether - Aminopyrimidine - Halopyrimidine - Aryl bromide - Aryl halide - Monocyclic benzene moiety - Benzenoid - Pyrimidine - Heteroaromatic compound - Carboxamide group - Secondary carboxylic acid amide - Carboxylic acid derivative - Organoheterocyclic compound - Azacycle - Hydrocarbon derivative - Organic oxide - Amine - Organic nitrogen compound - Organohalogen compound - Organobromide - Organonitrogen compound - Aromatic heteromonocyclic compound
• Description: This compound belongs to the class of organic compounds known as diarylethers. These are organic compounds containing the dialkyl ether functional group, with the formula ROR', where R and R' are aryl groups.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Associated Targets(Human)

ULK1 Tchem Serine/threonine-protein kinase ULK1 (0 Activities)

Discover Target: ULK1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

ULK2 Tchem Serine/threonine-protein kinase ULK2 (1 Activities)

Discover Target: ULK2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HL-60 (67320 Activities)

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Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MCF7 (126967 Activities)

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Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MDA-MB-468 (9477 Activities)

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Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

U-937 (7138 Activities)

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Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

A549 (127892 Activities)

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Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

ULK2 Tchem Serine/threonine-protein kinase ULK2 (652 Activities)

Discover Target: ULK2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action

Chemical and Physical Properties

• Solubility: Solubility (25°C) In vitro DMSO: 97 mg/mL (198.23 mM); Ethanol: 20 mg/mL warmed with 50ºC Water: bath (40.87 mM); Water: Insoluble
• DMSO(mg / mL) Max Solubility: 97
• DMSO(mM) Max Solubility: 198.2342843
• Water(mg / mL) Max Solubility: ＜1
• Molecular Weight: 489.300 g/mol
• XLogP3: 3.800
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 8
• Rotatable Bond Count: 8
• Exact Mass: 488.07 Da
• Monoisotopic Mass: 488.07 Da
• Topological Polar Surface Area: 104.000 Ų
• Heavy Atom Count: 31
• Formal Charge: 0
• Complexity: 560.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

References

1. Lin Liu, Zhuangzhuang Chu, Xiao Han, Jin Wu, Kunzhan Cai, Jiaohong Wang, Zixiang Guo, Shan Gao, Guoqing Li, Chunbo Tang.  (2025)  Creatine promotes osteogenic differentiation of dental pulp stem cells via the AMPK-ULK1-autophagy axis.  CONNECTIVE TISSUE RESEARCH,      [PMID:39930963] [10.1080/03008207.2025.2459243]