GRADE & PURITYSuitable for molecular biology?Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents.BioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.PCR Reagent?PCR grade — nuclease-free, inhibitor-free reagents for reliable amplification. Use in PCR/qPCR where contaminants would block or bias amplification.for DNA synthesis?DNA-synthesis grade — high purity reagents for oligonucleotide synthesis. Use in phosphoramidite DNA synthesis where purity drives yield.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.For In Vitro Transcription?In-vitro transcription grade — nuclease-free reagents for RNA synthesis in vitro. Use to make RNA transcripts/probes free of degradation.for DNA and RNA applications?For nucleic-acid (DNA & RNA) applications — nuclease-controlled across both. Use in workflows handling DNA and RNA together where degradation is a risk.10 U/μL
BioReagent, PCR Reagent, for DNA synthesis, Suitable for molecular biology, EnzymoPure™, For In Vitro Transcription, for DNA and RNA applications, 10 U/μL BioReagent,for DNA and RNA applications,for DNA synthesis,For In Vitro Transcription,PCR Reagent,Suitable for molecular biology,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
🌡
Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
📋
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
📚
Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
T767021
Component
250 U
1000 U
Storage
T767021A
TelN Protelomerase
25 μL
100 μL
-20°C. Avoid freeze/thaw cycle
T767021B
10×TelN Reaction Buffer
250 μL
1 mL
-20°C. Avoid freeze/thaw cycle
TelN Protelomerase, whose Chinese name is TelN Prokaryotic telomerase, is cloned from phage N15, which cuts dsDNA at the recognition site TelRL (56bp), The TelRL site consists of a palindromic sequence TelO in the middle and a palindromic sequence R3 and L3 at both ends of 14bp (Fig.1), and leaves a covalently closed end at the cleavage site, thus effectively converting circular DNA into linear DNA with hairpin ends (Fig.2). Source E.coli Enzyme Activity Definition The amount of enzyme required to completely cut 0.5 μg linearized pMiniT-TelN control plasmid (313 fmol TelN recognition site) for 30 min in a 50 μl system at 30°C was defined as 1 unit of activity (U). Protocol (1) Configure the mixture as shown in the following table:
Component
volume
dsDNA
X μl
10×TelN Reaction Buffer
2 μl
TelN Protelomerase
1 μl
Nuclease-free Water
To 20 μl
(2) Shake the mixed system gently up and down, and centrifuge briefly. (3) Incubate at 30℃ for 30 min. (4) Incubation at 75℃ for 5 min terminated the reaction. Cautions (1) This product is for scientific research purposes only and shall not be used for other purposes (since 2022.05.31). (2) In some application scenarios, there are patent restrictions on the use of this enzyme.
Specifications
Product Name
TelN Protelomerase
Synonyms
TelN Protelomerase
Grade
BioReagent, for DNA and RNA applications, for DNA synthesis, For In Vitro Transcription, PCR Reagent, Suitable for molecular biology, EnzymoPure™
Specifications & Purity
BioReagent, PCR Reagent, for DNA synthesis, Suitable for molecular biology, EnzymoPure™, For In Vitro Transcription, for DNA and RNA applications, 10 U/μL
Molecule Type
Enzyme
Storage and Shipping
Concentration
10 U/μL
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
ZJ25F0521466