GRADE & PURITYBioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.≥90%(SDS-PAGE)≥0.1 U/μL
Accession #
A0A1J0LV32
Expression system
E. coli
Bioactivity
≥ 0.1 U/μ L, unit definition: 1 active unit (U) refers to the amount of enzyme required to release 1.0 μ mol of inorganic orthophosphate per minute at pH 7.2 and 25 ℃ for 30 minutes.
ActiBioPure™, Bioactive, High Performance, EnzymoPure™
Specifications & Purity
EnzymoPure™, ActiBioPure™, Bioactive, High Performance, ≥90%(SDS-PAGE), ≥0.1 U/μL
Biochemical and Physiological Mechanisms
Catalyzes the hydrolysis of inorganic pyrophosphate (PPi) forming two phosphate ions.
Bioactivity
≥ 0.1 U/μ L, unit definition: 1 active unit (U) refers to the amount of enzyme required to release 1.0 μ mol of inorganic orthophosphate per minute at pH 7.2 and 25 ℃ for 30 minutes.
1.Di Wu, Lingxia Qin, Yeping Cen, Jin Lu, Shaohua Ma, Yufang Hu. (2024) Advanced electrochemical detection of pyrophosphatase activity in LNCaP cells utilizing in-situ redox of copper. MICROCHEMICAL JOURNAL, [PMID:][10.1016/j.microc.2024.111808]
2.Rao Honghong, Li Jianying, Luo Mingyue, Zhang Kehui, Gou Hao, Liu Haixia, Xue Zhonghua. (2022) A label-free and modification-free ratiometric electrochemical strategy for enhanced natural enzyme detection using a bare electrode and nanozymes system. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 414 (9):(2991-3003). [PMID:35106612][10.1007/s00216-022-03932-9]
3.Mengke Wang, Lei Wang, Qing Liu, Xingguang Su. (2017) A fluorescence sensor for protein kinase activity detection based on gold nanoparticles/copper nanoclusters system. SENSORS AND ACTUATORS B-CHEMICAL, [PMID:][10.1016/j.snb.2017.09.213]
4.Jingjing Yin, Yuehua Wang, Shanshan Zhu, Xiaoxue Liu, Yujie Sun, Hong Yang, Yifei Ma, Jinhua Liu. (2026) HRP-induced wide wavelength-tunable in situ fluorogenic system and its immunoassay for cTnI in clinical samples. TALANTA, [PMID:41691890][10.1016/j.talanta.2026.129511]
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