Tricolor Prestained Protein MW Marker (9-180kDa) - for western blot, suitable for electrophoresis, 9-180kDa; 9 Green/ 16 Blue/ 25 Blue/ 30 Blue/ 38 Blue/ 50 Blue/ 64 Orange/ 85 Blue/ 125 Blue/ 180 Blue kDa

Cat. No.: T1491646

GRADE & PURITY Suitable for electrophoresis, for Western blot, 9-180kDa; 9 Green/ 16 Blue/ 25 Blue/ 30 Blue/ 38 Blue/ 50 Blue/ 64 Orange/ 85 Blue/ 125 Blue/ 180 Blue kDa

Synonyms

Prestained Color Protein Marker | Prestained Color Protein Ladder | Broad Multi Color Pre-Stained Protein Standard | Star Ribbon Pre-stained Protein Marker | Tricolor Pre-Stained Protein Marker

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Application

WB

Overview

This product consists of 10 protein bands ranging from 9 kDa to 180 kDa (9 kDa, 16 kDa, 25 kDa, 30 kDa, 38 kDa, 50 kDa, 64 kDa, 85 kDa, 125 kDa, 180 kDa). The low-to-high molecular weight range allows monitoring of protein separation during electrophoresis, determination of target protein molecular weights, and assessment of transfer efficiency in Western Blot experiments. The 9 kDa and 64 kDa bands are conjugated with green or red dyes, facilitating real-time observation of the electrophoresis process and determination of protein molecular weights.

Usage method:

1. Place at room temperature for several minutes before loading. After thawing, gently shake to ensure the solution is thoroughly mixed.

2. Load an appropriate volume into the gel wells. The recommended volume is 2–5 μL per well, which can be adjusted according to specific conditions.

3. After use, cap the tube and return it to 4°C or -20°C.

Note: Taking a 5 μL loading volume as an example, the protein content of each band is approximately 1–3 μg. 

Precautions

1. This product already contains loading buffer. Use it directly; do not heat or boil.

2. To avoid repeated freeze-thaw cycles and contamination, aliquot the product and store at -20°C.

3. The prestained protein molecular weight standard is intended only for approximate molecular weight reference; batch-to-batch variation is approximately 5%.

4. In low-concentration gels, low molecular weight bands may co-migrate with the dye front and cannot be resolved.

5. The migration of prestained proteins may vary in different gel and buffer systems. Calibration using a non-prestained protein molecular weight standard is recommended, and the product can be used normally after calibration.

6. The protein molecular weight standard may exhibit non‑specific binding to antibodies. This can be optimized by reducing antibody concentration, shortening incubation time, and decreasing the loading amount of the protein molecular weight standard.

7. For your safety and health, please wear a lab coat and disposable gloves during handling.

8. This product is for research use only.

Specifications

Synonyms

Prestained Color Protein Marker | Prestained Color Protein Ladder | Broad Multi Color Pre-Stained Protein Standard | Star Ribbon Pre-stained Protein Marker | Tricolor Pre-Stained Protein Marker

Specifications & Purity

for western blot, suitable for electrophoresis, 9-180kDa; 9 Green/ 16 Blue/ 25 Blue/ 30 Blue/ 38 Blue/ 50 Blue/ 64 Orange/ 85 Blue/ 125 Blue/ 180 Blue kDa

Stability And Storage

Store at 2-8℃ short term (3 months). Store at -20℃ long term (24 months). Avoid freeze/thaw cycle. Upon receipt, it is recommended to aliquot.

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

for Western blot, Suitable for electrophoresis

Images

SDS-PAGE band profile in Bis-Tris: Tricolor Prestained Protein MW Marker (9-180kDa) (T1491646)

Tricolor Prestained Protein MW Marker (9-180kDa) (T1491646) - SDS-PAGE band profile in Bis-Tris
lmages are from a Bis-Tris gel (SDS-PAGE) and subsequent transfer to membrane. Tricolor Prestained Protein MW Marker (9-180kDa) (T1491646) are mixture of 10 recombinant proteins, 8 blue-stained bands, a green-stained band (9kDa) and an orange-stained band (64kDa).

SDS-PAGE band profile in Tris-Glycine: Tricolor Prestained Protein MW Marker (9-180kDa) (T1491646)

Tricolor Prestained Protein MW Marker (9-180kDa) (T1491646) - SDS-PAGE band profile in Tris-Glycine
lmages are from a Tris-glycine gel (SDS-PAGE) and subsequent transfer to membrane. Tricolor Prestained Protein MW Marker (9-180kDa) (T1491646) are mixture of 10 recombinant proteins, 8 blue-stained bands, a green-stained band (9kDa) and an orange-stained band (64kDa).