UltraBio™ Rabbit IgG Magnetic Beads

Cat. No.: R751580

Overview

Aladdin's UltraBio™ Rabbit IgG Magnetic Beads are based on amino magnetic beads, with 200nm particle size, covalently coupled with high-quality rabbit IgG antibody, generally used as the negative control of magnetic beads coupled with rabbit antibody against tags or target proteins in immunoprecipitation IP), co-immunoprecipitation (Co-IP), and chromatin immunoprecipitation (ChIP) assays.The rabbit IgG coupled with the Magnetic Beads is a normal, non-specific rabbit IgG without any labeling.UltraBio™ Rabbit IgG Magnetic Beads are used as the negative control for IP assays to address whether IgG itself is a source of non-specific binding. Please refer to Figure 1 for the workflow of IP assays with UltraBio™ Magnetic Beads.Figure 1. Workflow of IP assays with UltraBio™ Magnetic Beads.The UltraBio™ Rabbit IgG Magnetic Beads have lower non-specificity and smaller particular size, compared to most other similar products. Per milliliter of the magnetic bead suspension contains 10mg of magnetic beads coupled with no less than 0.5mg of rabbit IgG antibody.The UltraBio™ Rabbit IgG Magnetic Beads are convenient to use. Magnetic Beads are kept in a special protective solution without glycerol, and can be quickly and efficiently separated from the solution manually by using a magnetic stand or automatically by using an instrument.Main features of the UltraBio™ Rabbit IgG Magnetic Beads

Precautions：

Repeated freezing and thawing of this product up to 3 times does not cause obvious changes in its performance.The pH of Rabbit IgG Magnetic Beads should be maintained at 6-8. Do not centrifuge, dry, or freeze the magnetic beads. Long-time exposure of the beads to a magnetic field will cause the bead to aggregate. We recommend including both negative and positive controls when performing IP assays.Protein samples should be purified after collection as soon as possible and kept at 4℃ or on ice to minimize protein degradation or denaturation. Protein degradation can also be inhibited by adding appropriate protease inhibitors (, P1005/P1006, P1048/P1049, P1010/P1011, P1050/P1051).If using a vacuum pump to aspirate supernatant, the strength of the vacuum pump should be controlled properly to avoid the aspiration of magnetic beads.It is normal to see magnetic beads aggregate when an acidic solution is used for the elution, which can be prevented effectively by containing 0.1% non-ionic detergent (such as Triton X-100, Tween-20, or NP-40) without affecting the performance of this product.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1. Preparation of protein samplesa. Lysis cells or tissues with appropriate lysis buffer. We recommend using Rabbit IgG Magnetic Beads used as the negative control are eluted in the same manner.(a) Preparation of 3X Flag elution buffer. Dissolve 3X Flag Peptide ( Protein A Magnetic Beads1mlP2102-5ml Protein A Magnetic Beads5mlP2105-1ml Protein G Magnetic Beads1mlP2105-5ml Protein G Magnetic Beads5mlP2108-1ml Protein A+G Magnetic Beads1mlP2108-5ml Protein A+G Magnetic Beads5mlP2115-0.5ml Anti-Flag Magnetic Beads0.5mlP2115-2ml Anti-Flag Magnetic Beads2mlP2118-0.5ml Anti-Myc Magnetic Beads0.5mlP2118-2ml Anti-Myc Magnetic Beads2mlP2121-0.5ml Anti-HA Magnetic Beads0.5mlP2121-2ml Anti-HA Magnetic Beads2mlP2151-1ml Streptavidin Magnetic Beads1mlP2151-200μl Streptavidin Magnetic Beads200μlP2151-5ml Streptavidin Magnetic Beads5mlP2171-1ml Mouse IgG Magnetic Beads1mlP2171-5ml Mouse IgG Magnetic Beads5ml

Specifications

Product Name

UltraBio™ Rabbit IgG Magnetic Beads

Molecule Type

Bioreagents/Buffer

Storage and Shipping

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 4℃ for up to 2 years, or at -20℃ for long-term storage.