Vicia ervilia lectin or agglutinin (VEA) is isolated from bitter vetch seeds and has a molecular weight of 53 kDa. This lectin is blood group non-specific with sugar specificity for α-mannose, and to a lesser extent, α-glucose residues. Agglutination is inhibited by mannose, glucose, fructose, methyl mannoside, maltose, melezitose and α, α’-trehalose.
Aladdin’s VEA lectin is supplied as a yellow lyophilized powder without preservatives. The purity of VEA is determined by SDS-PAGE.
Benefits
• Ultrapure quality
• Sugar specificity: α-Mannose > α-Glucose
• Non-specific blood group agglutination
• Lyophilized powder
Applications
• Membrane protein studies
Specifications
| Parameter | Details |
|---|
| Appearance | Yellow lyophilized powder or flocculate
| | Source | Vicia ervilia
| | Molecular weight | 53 kDa
| | Sugar specificity | α-Man > α-Glu
| | Activity | Agglutinates rabbit erythrocytes at <8 μg/ml, Agglutination is inhibited by 10mM D-mannose at 4x agglutination titer.
| | Microorganisms | ≤ 100 CFU/g | | Shelf life | ≥ Three years when stored at -20°C |
|
Directions for use
The lectin may be reconstituted with 2 ml of deionized water before use, spin the vial gently until full dissolution. The solution may be reconstituted in this buffer to desired working concentration.
Tips and hints
Avoid repeated freezing and thawing.
Shipping and storage
The product is shipped at -20°C however for over-the-day transport it may be shipped at ambient temperature. The lyophilized powder is stable for more than three years from production date when stored below -20°C. After reconstitution with deionized water, the solution may be stored frozen in working aliquots for up to 12 months.
ZJ26F0435011