Western Rapid Transfer Buffer (20X, Wet) - 20X

Cat. No.: T751592

GRADE & PURITY 20X

Synonyms

Transfer Buffer | Fast Transfer buffer

Storage

Room temperature

Shipped In

Normal

Overview

Product Introduction:

This product adopts a new rapid transfer formula, which can complete Western Blot wet transfer within 15-40 minutes, with low heat generation and no need for an ice bath. Dilute the transfer solution and use it directly, compatible with methanol and ethanol. Compatible with Tris Gly system, Hepes system, Bis Tris system and other self-adhesive and prefabricated adhesives, compatible with PVDF membrane and NC membrane.

Precautions：

1. If the room temperature is high during the experiment, an ice box can be used to cool down the transfer tank without the need for an ice bath.
2. PVDF membrane should be activated with anhydrous ethanol or methanol before use.

Instructions for Use：

Preparation of 1000 mL rapid membrane transfer solution (1 ×) (both methods have the same effect, please choose according to usage habits):

 

Component	Method 1	Method 2
Western Rapid Transfer Buffer (20×)	50mL	50mL
Deionized water	850mL	900mL
Absolute alcohol(or methanol)	100mL	50mL

Film transfer conditions：

1. The transfer efficiency is related to the gel concentration. For various concentrations of 1.0 mm PAGE gel, a constant current of 400 mA can be used for the following duration to completely transfer proteins below 150 kDa. If the molecular weight is greater than 150 kDa, the transfer time needs to be extended by 5-10 minutes：

Recommended duration	6%	8%	10%	12%	15%
Recommended duration	15min	20min	30min	35min	40min

 

 

2. For 1.5 mm PAGE gel, the transfer time in the above table can be extended by 5-10 minutes accordingly.

Specifications

Synonyms

Transfer Buffer | Fast Transfer buffer

Specifications & Purity

20X

Storage

Room temperature

Shipped In

Normal

Note

This product is about to be sold out and removed from the shelves. The replacement product is W1515787.