Wheat Germ Agglutinin (WGA), AF488 conjugate, CAS No.96508-08-6

CAS: 96508-08-6 Cat. No.: np231283

GRADE & PURITY BioReagent, Suitable for Immunofluorescence(IF), 1.0 mg/mL

Overview

Triticum vulgaris lectin or Wheat germ agglutinin (WGA) is isolated from Triticum vulgaris (Wheat germ) and purified by affinity chromatography. The lectin has two subunits and a molecular weight of 36 kDa. WGA selectively binds to N-Acetyl glucosamine (GlcNAc) and to N-acetylneuraminic acid (sialic acid) residues of glycoproteins and glycolipids, which are often found on cell membranes.

WGA is commonly used to label glycoproteins for imaging of the plasma membrane in live or fixed cells, for staining of tissue sections, or for western blotting.  WGA can be used as a gram stain to fluorescently label gram bacteria but not gram- bacteria. WGA also binds to the bud scars on budding yeast such as Saccharomyces cerevisiae.

AF488 labeled WGA has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 490 nm and the emission maximum is at 525 nm. Fluorescent WGA conjugates provide selective labeling of the plasma membrane with minimal background in many cell types that is retained after formaldehyde fixation and permeabilization with Triton X-100.

Specifications

Product Name

Wheat Germ Agglutinin (WGA), AF488 conjugate, CAS No.96508-08-6

Synonyms

WGA, AF488 conjugate | Lectin from Triticum vulgaris (wheat), AF488 conjugate | Wheat Germ Agglutinin (WGA), F488 | AF488, WGA | AF488-Wheat germ agglutinin | WGA-AF488

Grade

BioReagent, Suitable for Immunofluorescence(IF)

Specifications & Purity

Suitable for Immunofluorescence(IF), BioReagent, 1.0 mg/mL

Conjugation

AF488

Excitation (nm)

Blue Laser(488nm)

Emission(nm)

525nm

Ex/Em(nm)

CAS

96508-08-6

Molecule Type

Protein

Storage and Shipping

Concentration

1.0 mg/mL

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

IF/ICC: Wheat Germ Agglutinin (WGA), AF488 conjugate (np231283)

Wheat Germ Agglutinin (WGA), AF488 conjugate (np231283) - IF/ICC 

HeLa cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Wheat Germ Agglutinin (WGA), AF488 conjugate (np231283) (green) at 1/200 dilution in blocking buffer overnight at 4°C in the dark. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Application

• Application: Dilution info
• IF/ICC:
  1/10-1/200

References

1. Qian Wang, Zichao Peng, Bin Gui, Yongming Li, Lanjie Liao, Zuoyan Zhu, Yaping Wang, Libo He.  (2025)  Sialic acid serves as a functional receptor for grass carp reovirus.  PLoS Pathogens,  21  (9): (e1013481).  [PMID:40911632] [10.1371/journal.ppat.1013481]

2. Donghua Su, Xiaolong Zhang, Tianwu An, Xi Wang, Yaqiu Lin, Haibo Wang, Peiqiang Yu, Haitao Shi.  (2025)  Astral-DIA proteomics and 4D label-free N-glycoproteomics of milk fat globule membrane proteins reveal distinct profiles between yak colostrum and mature milk.  Food Chemistry-X,      [PMID:41378274] [10.1016/j.fochx.2025.103302]