X-Gal Solution (20 mg/mL) - BioReagent , CAS No.7240-90-6

CAS: 7240-90-6 Cat. No.: X1520260 Molecular Weight: 408.63 Beilstein Registry Number: 1402009 EC Number: 230-640-8

GRADE & PURITY BioReagent

Synonyms

5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Enzyme activity assay, Reporter Gene Assay

Overview

X-Gal is an inert chromogenic substrate for β-galactosidase. It is colorless itself, but upon hydrolysis by β-galactosidase, it produces colorless galactose and 4-chloro-3-bromo-indigo, which forms a deep blue precipitate, facilitating visual observation or instrument detection. IPTG can induce the expression of the lacZ gene, thereby promoting the hydrolysis of X-Gal by β-galactosidase, resulting in the formation of blue colonies. It is commonly used for in situ staining detection of β-galactosidase and for blue-white screening.

Main Applications

• Blue-White Colony Screening: Used to distinguish recombinant colonies (white) from non-recombinant colonies (blue). It is a commonly used screening method in molecular cloning.

• Eukaryotic Cell Detection: Enables visualization of β-galactosidase reporter gene expression in transfected eukaryotic cells, providing a direct reflection of reporter gene expression levels.

• Activity Assay: Used for detecting β-galactosidase activity in immunological and histochemical procedures, aiding research into related molecular mechanisms.

Instructions (For Reference Only)

Operate according to the specific requirements of the experiment.

Precautions

1. During storage and use of the solution, avoid repeated freeze-thaw cycles to prevent affecting product activity and stability.

2. To ensure the safety and health of experimental personnel, please wear a laboratory coat and disposable gloves during operation to avoid direct skin contact.

Specifications

Synonyms

5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside

Specifications & Purity

BioReagent

Stability And Storage

Store at -20℃ long term (12 months). Store in the dark. Avoid freeze/thaw cycle.

Storage

Protected from light, Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent

Names and Identifiers

• Isomeric SMILES: C1=CC(=C(C2=C1NC=C2O[C@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O)Cl)Br
• WGK Germany: 3
• Molecular Weight: 408.63
• Beilstein: 1402009
• Reaxy-Rn: 32869881
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=32869881&ln=

Chemical and Physical Properties

• Sensitivity: Light-sensitive
• Specific Rotation[α]: -66 ° (C=1, DMF)
• Melt Point(°C): 236°C

References

1. Chunxu Chen, Tianhui Li, Guijie Chen, Dan Chen, Yujia Peng, Bing Hu, Yi Sun, Xiaoxiong Zeng.  (2020)  Commensal Relationship of Three Bifidobacterial Species Leads to Increase of Bifidobacterium in Vitro Fermentation of Sialylated Immunoglobulin G by Human Gut Microbiota.  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,      [PMID:32806107] [10.1021/acs.jafc.0c03628]

2. Zhi-P. Cai, Louis P. Conway, Ying Y. Huang, Wen J. Wang, Pedro Laborda, Ting Wang, Ai M. Lu, Hong L. Yao, Kun Huang, Sabine L. Flitsch, Li Liu, Josef Voglmeir.  (2019)  Enzymatic Synthesis of Trideuterated Sialosides.  MOLECULES,  24  (7): (1368).  [PMID:30965582] [10.3390/molecules24071368]

3. Xiaochong Zhu, Jieyuan Wu, Shizhong Li, La Xiang, Jian-Ming Jin, Chaoning Liang, Shuang-Yan Tang.  (2024)  Artificial Biosynthetic Pathway for Efficient Synthesis of Vanillin, a Feruloyl-CoA-Derived Natural Product from Eugenol.  JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,      [PMID:38501643] [10.1021/acs.jafc.3c08723]

4. Zi-Xuan Hu, Shu-Rui Li, Qing-Jun Xia, Ting Wang, Josef Voglmeir, Göran Widmalm, Li Liu.  (2024)  Enzymatic synthesis of N-formylated sialosides via a five-enzyme cascade.  ORGANIC & BIOMOLECULAR CHEMISTRY,  22  (36): (7485-7491).  [PMID:39189395] [10.1039/D4OB00874J]

5. Xiaoyu Cui, Liqing Feng, Jiayin Li, Shan Gao, Shunping Yan, Ting Pan.  (2026)  Chloroplast vesiculation fine-tunes seed germination by antagonizing ABA signaling through dual degradation of ABI5 and XIW1.  PLANT JOURNAL,  125  (1): (e70669).  [PMID:41525299] [10.1111/tpj.70669]