Alcohol Oxidase, CAS No.9073-63-6

CAS: 9073-63-6 Cat. No.: A1491983 Molecular Weight: 191.31 EC Number: 232-971-3 PubChem CID: 73555276

GRADE & PURITY Native, EnzymoPure™, ≥20U/mg protein, ≥10U/mg powder; from Pichia pastoris

Accession #

P04842

Bioactivity

≥20U/mg protein, ≥10U/mg powder

Overview

In enzymology, an alcohol oxidase (EC 1.1.3.13) is an enzyme that catalyzes the chemical reaction:a primary alcohol + O₂↔ an aldehyde + H₂O₂. Thus, the two substRates of this enzyme are primary alcohol and O2, whereas its two products are aldehyde and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. It employs one cofactor, FAD.
Applications
Alcohol Oxidase may be used to study protein translocation into peroxisomes. This product is from Pichia pastoris. It has been used for the bacterial expression and immunological verification of Hv-p68 cDNA clones.

Specifications

Product Name

Alcohol Oxidase, CAS No.9073-63-6

Synonyms

Alcohol:oxygen oxidoreductase | alcohol oxidase | AOX | methanol oxidase | Alcohol oxidase 1 | AOX1

Grade

Native, EnzymoPure™

Specifications & Purity

Native, EnzymoPure™, ≥20U/mg protein, ≥10U/mg powder; from Pichia pastoris

Biochemical and Physiological Mechanisms

Major isoform of alcohol oxidase, which catalyzes the oxidation of methanol to formaldehyde and hydrogen peroxide, the first step in the methanol utilization pathway of methylotrophic yeasts.

Bioactivity

≥20U/mg protein, ≥10U/mg powder

Accession #

P04842

CAS

9073-63-6

Enzyme Commission Number

EC 1.1.3.13

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥20U/mg protein, ≥10U/mg powder; from Pichia pastoris

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 2-8℃ (1 years). Store at -20℃ long term (3 years). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

One unit will oxidize 1.0 μmole of methanol to formaldehyde per min at pH 7.5 at 25°C.

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic acids and derivatives
    • Class Carboxylic acids and derivatives
      • Subclass Amino acids, peptides, and analogues
        • Level5 Amino acids and derivatives
          • Level6 Alpha amino acids and derivatives
            • Level7 Alpha amino acids

• Kingdom: Organic compounds
• Superclass: Organic acids and derivatives
• Class: Carboxylic acids and derivatives
• Subclass: Amino acids, peptides, and analogues
• Intermediate Tree Nodes: Amino acids and derivatives - Alpha amino acids and derivatives
• Direct Parent: Alpha amino acids
• Alternative Parents: Amino acids Monocarboxylic acids and derivatives Carboxylic acids Organic oxides Monoalkylamines Hydrocarbon derivatives Carbonyl compounds Saturated hydrocarbons
• Molecular Framework: Not available
• Substituents: Alpha-amino acid - Amino acid - Carboxylic acid - Monocarboxylic acid or derivatives - Organic oxygen compound - Hydrocarbon derivative - Primary amine - Organooxygen compound - Organonitrogen compound - Organic nitrogen compound - Primary aliphatic amine - Carbonyl group - Amine - Organic oxide - Hydrocarbon - Saturated hydrocarbon - Aliphatic acyclic compound
• Description: This compound belongs to the class of organic compounds known as alpha amino acids. These are amino acids in which the amino group is attached to the carbon atom immediately adjacent to the carboxylate group (alpha carbon).
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

References

1. Anna Kharkova, Lyubov Kuznetsova, Roman Perchikov, Maria Gertsen, Pavel Melnikov, Nikolay Zaitsev, Jun Zhang, Vyacheslav Arlyapov.  (2025)  Bionanocomposite Four-Channel Biosensor for Rapid and Convenient Monitoring of Glucose, Lactate, Ethanol and Starch.  Gels,  11  (5): (355).  [PMID:40422375] [10.3390/gels11050355]