10mM in DMSO for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -80°C Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 3 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Information
Diphenhydramine HCl is a first-generationhistamine H1 receptorantagonist, used in various allergic conditions such as rhinitis, urticaria and conjunctivitis.
In vitro
Diphenhydramine blocks tetrodotoxin-sensitive (TTX-S) and tetrodotoxin-resistant (TTX-R) sodium currents with K(d) values of 48 mM and 86 mM, respectively, at a holding potential of -80 mV. Diphenhydramine shifts the conductance-voltage curve for TTX-S sodium currents in the depolarizing direction but has little effect on that for TTX-R sodium currents. Diphenhydramine causes a shift of the steady-state inactivation curve for both types of sodium currents in the hyperpolarizing direction. Diphenhydramine produces a profound use-dependent block when the cells are repeatedly stimulated with high-frequency depolarizing pulses. Diphenhydramine induces apoptosis in a dose- and time-dependent manner in both CCRF-CEM and Jurkat cell lines, whereas Cimetidine fails to induce significant effects at similar concentrations. Diphenhydramine-induced apoptosis is evaluated in terms of morphology, flow cytometry, and the release of cytochrome c to the cytosol. Diphenhydramine inhibits cell proliferation without inducing apoptosis in human peripheral blood mononuclear cells. Diphenhydramine (500 nM) significantly reduces the baseline firing of the periaqueductal gray neurons without a significant effect on the frequency of postsynaptic potentials. Diphenhydramine at high concentration inhibits periaqueductal gray neurons, but at low concentrations it has no effect on the baseline-firing rate and it blocks the response to neurotensin and tomedial preoptic nucleus stimulation.
In vivo
Cell Data
cell lines：
Concentrations：
Incubation Time：
Powder Purity:≥99%
| Isomeric SMILES | CN(C)CCOC(C1=CC=CC=C1)C2=CC=CC=C2.Cl |
|---|---|
| WGK Germany | 3 |
| RTECS | KR7000000 |
| Molecular Weight | 291.82 |
| Reaxy-Rn | 3918132 |
| Reaxys-RN_link_address | https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=3918132&ln= |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Solubility | Solubility (25°C) In vitro DMSO: 59 mg/mL (199.77 mM); |
|---|---|
| Melt Point(°C) | 170°C |
| 1. Jiezhou Pan, Haotian Liao, Guidong Gong, Yunxiang He, Qin Wang, Lang Qin, Yaoyao Zhang, Hirotaka Ejima, Blaise L. Tardy, Joseph J. Richardson, Jiaojiao Shang, Orlando J. Rojas, Yong Zeng, Junling Guo. (2023) Supramolecular nanoarchitectonics of phenolic-based nanofiller for controlled diffusion of versatile drugs in hydrogels. JOURNAL OF CONTROLLED RELEASE, [PMID:37422124] [10.1016/j.jconrel.2023.07.003] |
| 2. Yanru Liu, Yushan Ding, Yongbo Song, Xingjie Guo. (2021) Enantioseparation and determination of orphenadrine in rat plasma and its application to a stereoselective pharmacokinetic study. NEW JOURNAL OF CHEMISTRY, 45 (12): (5428-5436). [PMID:] [10.1039/D0NJ06005D] |
| 3. Yongjie Lu, Lushi Cheng, Lu Ren, Dongqiu Chen, Shumin Guan, Siyang Zhu, Xian Xu, Bing Zhang, Minghui Tang, Chijian Zhang, Yong Ai, Lanyue Zhang, Tinggang He. (2024) Therapeutic Alleviation and Mechanism of Glabridin Liposome on Histamine-induced Atopic Dermatitis. Pharmacognosy Magazine, [PMID:] [10.1177/09731296231225512] |
