Dissolved amino acids: how to prepare a solution with 1 equivalent of NaOH?
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Dissolved amino acids: how to prepare a solution with 1 equivalent of NaOH?
·Reviewed·Published ·Updated ·8 min read
Dissolved amino acids: how to prepare a solution with 1 equivalent of NaOH
Amino acids are often difficult to solubilize. A common technique is to use 1 molar equivalent (1eq) of sodium hydroxide (NaOH).
To dissolve compound in 1 equivalent of NaOH(aq.), the following formula can be used to calculate the volume of NaOH (aq) (VolNaOH) of concentration (ConcNaOH ) needed:
e.g for a product with a molecular weight of 197.13g, a mass of 5 mg, and using 100 mM NaOH:
Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
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Aladdin Scientific. "Dissolved amino acids: how to prepare a solution with 1 equivalent of NaOH?" Aladdin Knowledge Base, updated Jul 27, 2023. https://www.aladdinsci.com/us_en/faqs/dissolved-amino-acids-aladdin-en.html
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