GRADE & PURITYSterile-filtered?Sterile-filtered — passed through a 0.2µm filter to remove microorganisms. Use as a ready aseptic solution for culture and sensitive applications.BioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.Ready-to-use?Ready-to-use — supplied pre-formulated at working concentration, no prep needed. Use to save time and reduce pipetting/dilution errors.Sterile?Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization.Suitable for Immunohistochemistry(IHC)?IHC grade — validated for immunohistochemistry on tissue sections. Use to detect and localize antigens in fixed tissue.
sterile-filtered, BioReagent, ready-to-use, Suitable for Immunohistochemistry, sterile BioReagent,Ready-to-use,Sterile,Sterile-filtered,Suitable for Immunohistochemistry(IHC) for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Description This product is an optimized antibody diluent for immunostaining on the basis of traditional secondary antibody diluent, which can be used for the dilution of secondary antibody in non-fluorescent immunostaining such as immunohistochemical and immunocytochemical. Through the improvement of the detergent and the stabilizer of the second antibody, the product not only effectively reduces the non-specific binding of the second antibody, but also improves the stable preservation time of the second antibody after dilution. Procedure : 1. Reagent preparation refer to the instructions for the use of the second antibody used, as well as the quality of the primary antibody and the content of the target protein in the sample, dilute the second antibody in accordance with the appropriate proportion, such as 1:1000, 1:500 and other proportions. 2. Antibody incubation The second antibody can be incubated at the appropriate temperature after dilution of this product, and is directly used for non-fluorescent immunostaining. 3. Color reaction according to the needs of the experiment, choose the appropriate color reagent for color development. Note: 1. This product has been filtered treatment, once started, it is recommended to use within one month, if found precipitate or bacterial contamination please discard. 2. The second antibody diluted by this product can be reused 3 to 5 times within 1 to 2 weeks under appropriate storage conditions. Tips: For your own safety, please take good protection before using the reagent, such as wearing lab clothes, gloves, etc.
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