Metallo-β-lactamase, CAS No.9073-60-3

CAS: 9073-60-3 Cat. No.: M1508747 EC Number: 232-970-8 PubChem CID: 133358

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, Sterile, expressed in E.coli;≥2M units/EA

Accession #

P14488

Expression system

E. coli

Overview

Metallo-β-lactamase, also known as imipenem hydrolase, is a high-purity antibiotic-inactivating enzyme produced by genetic engineering technology. It can hydrolyze the β-lactam ring of carbapenem antibiotics, thereby abrogating their antimicrobial activity.

This product is a highly purified and highly active preparation obtained through purification processes. It is characterized by strong specificity, capable of hydrolyzing the β-lactam ring of antibiotics such as imipenem, meropenem, and ertapenem. It also exhibits hydrolytic activity against cefixime, cefoperazone, cefotaxime, ceftriaxone, and other cephalosporins.

Scope of Application

It can be used for the sterility test and microbial limit test of carbapenem antibiotic raw materials and their preparations, namely the sterility test for β-lactam antibiotics specified in Pharmacopoeia of the People's Republic of China (2020 Edition, Volume 4, Appendix: Sterility Test) and the microbial limit test specified in the corresponding appendix. It is also used as a reagent for the sterility test specified in USP-NF General Chapters 71 STERILITY TEST.

Usage Instructions

Sterility Test Method for Carbapenem Antibiotics (with reference to Pharmacopoeia of the People's Republic of China (2020 Edition, Appendix: Sterility Test)): Membrane Filtration Method or Direct Inoculation Method

Specifications

Product Name

Metallo-β-lactamase, CAS No.9073-60-3

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, Sterile, EnzymoPure™

Specifications & Purity

ActiBioPure™, Bioactive, High Performance, EnzymoPure™, Recombinant, sterile, expressed in E.coli;≥2M units/EA

Biochemical and Physiological Mechanisms

Confers resistance to the different beta-lactams antibiotics (penicillin, cephalosporin and carbapenem) via the hydrolysis of the beta-lactam ring. Benzylpenicillin is a better substrate than cephalosporin C and ampicillin.

Accession #

P14488

CAS

9073-60-3

Enzyme Commission Number

EC 3.5.2.6

Molecule Type

Enzyme

Storage and Shipping

Concentration

expressed in E.coli;≥2M units/EA

Reconstitution

Reconstitute in sterile pre-chilled water.

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ long term (24 months). Upon reconstitution, it is recommended to aliquot.

Unit definition

The amount of enzyme required to hydrolyze 1 unit of penicillin G in one hour at pH 7.0 and 37˚C.

References

1. Maohu Chen, Bo Qiu, Zhanlin Zhang, Shuang Xie, Yuan Liu, Tian Xia, Xiaohong Li.  (2021)  Light-triggerable and pH/lipase-responsive release of antibiotics and β-lactamase inhibitors from host-guest self-assembled micelles to combat biofilms and resistant bacteria.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2021.130330]

2. Wenyao Chen, Shan Shan, Juan Peng, Daofeng Liu, Jun Xia, Bing Shao, Weihua Lai.  (2020)  Sensitive and hook effect–free lateral flow assay integrated with cascade signal transduction system.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2020.128465]

3. Shan Shan, Daofeng Liu, Qi Guo, Songsong Wu, Rui Chen, Kai Luo, Liming Hu, Yonghua Xiong, Weihua Lai.  (2016)  Sensitive detection of Escherichia coli O157:H7 based on cascade signal amplification in ELISA.  JOURNAL OF DAIRY SCIENCE,      [PMID:27394946] [10.3168/jds.2016-11320]

4. Chen Chen, Jun Lv, Wanyi Chen, Yang Xiang, Juan Zhang.  (2016)  Colorimetric β-lactamase inhibitor assay with double catalyzed signal amplification.  NEW JOURNAL OF CHEMISTRY,  40  (3): (1982-1987).  [PMID:] [10.1039/C5NJ02356D]

5. Lihong Li, Zhao Li, Wen Shi, Xiaohua Li, Huimin Ma.  (2014)  Sensitive and Selective Near-Infrared Fluorescent Off–On Probe and Its Application to Imaging Different Levels of β-Lactamase in Staphylococcus aureus.  ANALYTICAL CHEMISTRY,      [PMID:24844761] [10.1021/ac501288e]

6. Junfeng Liu, Liying Zhang, Haodi Ma, Haoyang Sun, Shu-ai Ge, Jieyi Liu, Shengdi Fan, Chunshan Quan.  (2024)  Quaternary ammonium chitosan-functionalized mesoporous silica nanoparticles: A promising targeted drug delivery system for the treatment of intracellular MRSA infection.  CARBOHYDRATE POLYMERS,      [PMID:39843087] [10.1016/j.carbpol.2024.123184]