PCNA Mouse mAb (AF594) - Primary antibody, specific to PCNA

Cat. No.: Ab219144
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 0.5 mg/mL
Application
IF/ICC
Host species
Mouse
Reactive species
Human, Mouse, Rat
Isotype
Mouse IgG1
Clone Number
C04/3D7
Conjugation
AF594
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab219144-10μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$59.90
50μl
Ab219144-50μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$169.90
100μl
Ab219144-100μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$259.90
1ml
Ab219144-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$1,599.90
Enter a quantity for the sizes you want to add.
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Why this grade

ExactAb™,Validated,0.5 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Protected from light,Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse anti Human PCNA Antibody, Monoclonal (C04/3D7), could be used for IF/ICC.

Specifications

Product Name
PCNA Mouse mAb (AF594) - Primary antibody, specific to PCNA
Synonyms
ATLD2 antibody | cb16 antibody | Cyclin antibody | DNA polymerase delta auxiliary protein antibody | etID36690.10 antibody | fa28e03 antibody | fb36g03 antibody | HGCN8729 antibody | MGC8367 antibody | Mutagen-sensitive 209 protein antibody | OTTHUMP00000
Specifications & Purity
ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms
Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple prote
Host species
Mouse
Specificity
PCNA
Immunogen
Full length recombinant human PCNA
Positive Control
IF/ICC: Hela cells
Conjugation
AF594
Excitation (nm)
Yellow-Green Laser(594nm)
Emission(nm)
618nm
Ex/Em(nm)
Ex:590nm;Em:618nm
Grade
ExactAb™, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
Images
PCNA Mouse mAb (AF594) (Ab219144) - IF/ICC 
IF analysis of PCNA (Red) in Hela cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with PCNA Mouse mAb (AF594) (Ab219144) at a dilution of 1/500 in blocking buffer overnight at 4°C. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
PCNA Tchem Proliferating cell nuclear antigen (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Solution Calculators
Reviews

Customer Reviews

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