GRADE & PURITYBioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.≥90%(SDS-PAGE)≥300U/mg enzyme powder; ≥500U/mg protein
Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), ≥300U/mg enzyme powder; ≥500U/mg protein ActiBioPure™,Bioactive,High Performance,Recombinant,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
3-Hydroxybutyrate Dehydrogenase (3-HBDH) is a mitochondrial enzyme, is often used in biochemical studies. 3-hydroxybutyrate dehydrogenase is involved in the synthesis and degradation of ketone bodies and butanoate metabolism. 3-Hydroxybutyrate dehydrogenase catalyzes (R)-3-hydroxybutanoate converts into acetoacetate.
Reaction
Product description
Appearance:
White amorphous powder
Source:
Microorganism
Enzyme Commission Number:
EC 1.1.1.30
CAS Number:
9028-38-0
Storage temperature:
-20℃
Activity:
≥300U/mg enzyme powder; ≥500U/mg protein
Unit definition:
One unit converts one micromole of 3-hydroxybutylate to acetoacetate per min at pH 8.5 at 37℃.
Properties
Stability:
Stable at -20℃ for at least five years
Molecular weight:
27kDa (SDS-PAGE)
Isoelectric point:
7.2
Michaelis constant:
2.1×10⁻³M (D-3-Hydroxybutyrate)
Optimum pH:
7.0~9.0
{Fig. 1}
Optimum temperature:
60℃
{Fig. 3}
pH Stability:
6.0~11.0 (25℃, 24hr)
{Fig. 2}
Thermal stability:
< 37℃ (pH 8.5, 30min)
{Fig. 4}
Inhibitors:
Cu²⁺, Fe³⁺, Zn²⁺, NEM, Proclin, SDS
Effect of various chemicals:
{Table 1}
Table 1. Effect of Various Chemicals on HBDH
[The enzyme dissolved in 50mM Tris-HCl buffer, pH 8.5 (20U/ml) was incubated with each chemical at 37℃ for 2hr.]
Specifications
Product Name
Recombinant 3-Hydroxybutyrate Dehydrogenase (HBDH), CAS No.9028-38-0
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