GRADE & PURITYBioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥10000U/mg protein
Accession #
Q8DR60
Protein Tag
N-His
Expression system
E. coli
Bioactivity
≥10,000 U/mg protein, unit definition: At 37℃ and pH 5.5 conditions, the amount of enzyme required to catalyze the release of 1 nmol of Gal β1-3GalNAcα1-Thr per hour.
ActiBioPure™, Bioactive, High Performance, His Tag, EnzymoPure™
Specifications & Purity
Bioactive, ActiBioPure™, High Performance, EnzymoPure™, His-Tag, ≥10000U/mg protein
Biochemical and Physiological Mechanisms
Releases unsubstituted Ser- and Thr-linked β-Gal-(1→3)-α-GalNAc (Core 1 type O-glycan) from glycoproteins. Substitutions of the disaccharide core with sialic acid, lactosamine (galactose-N-acetyl glucosamine), or fucose will block hydrolysis and prevent
Bioactivity
≥10, 000 U/mg protein, unit definition: At 37℃ and pH 5.5 conditions, the amount of enzyme required to catalyze the release of 1 nmol of Gal β1-3GalNAcα1-Thr per hour.
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