Ac-WEHD-AMC, TFA salt - BioReagent, ≥98% , CAS No.189275-74-9 (free base)

CAS: 189275-74-9 (free base) Cat. No.: A1456354 Molecular Weight: 784.8 (free base)

GRADE & PURITY BioReagent, ≥98%

Synonyms

Caspase-1 Substrate X, Fluorogenic | Caspase-5 Substrate III, Fluorogenic | Ac-Trp-Glu-His-Asp-AMC | Ac-Trp-Glu-His-{Asp-AMC}

Storage

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

Apoptosis, Cell signaling, Drug screening, Enzyme activity assay

Overview

Ac-WEHD-AMC is a potent fluorescent substrate for caspase-1.

Applications and Functions

1. Caspase-1 Enzyme Activity Detection

Ac-WEHD-AMC serves as a specific substrate for caspase-1 (a cysteine protease involved in the cell apoptosis process). When caspase-1 is present, it cleaves the peptide bond in Ac-WEHD-AMC, releasing the fluorescent group AMC. The enzyme activity of caspase-1 can then be detected by monitoring changes in fluorescence intensity. This method is widely used in research on biological processes such as cell apoptosis and inflammatory responses.

2. Drug Screening and Development

Due to the specific interaction between Ac-WEHD-AMC and caspase-1, it can be used to screen and develop inhibitors or agonists targeting caspase-1. Such compounds hold potential for treating diseases like neurodegenerative disorders and autoimmune diseases.

3. Biomedical and Molecular Biology Research

Ac-WEHD-AMC also functions as a tool compound in biochemical and molecular biological studies, aiding in the exploration of caspase-1’s roles and mechanisms in biological processes such as cell signaling and protein degradation.

Specifications

Synonyms

Caspase-1 Substrate X, Fluorogenic | Caspase-5 Substrate III, Fluorogenic | Ac-Trp-Glu-His-Asp-AMC | Ac-Trp-Glu-His-{Asp-AMC}

Specifications & Purity

BioReagent, ≥98%

Stability And Storage

Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark. Desiccated

Storage

Protected from light, Store at -20°C, Desiccated, Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent

Purity

≥98%

Names and Identifiers

• Molecular Weight: 784.8 (free base)

Chemical and Physical Properties

• Solubility: Soluble in 10% ACN/H2O.