GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Alanine aminotransferase (ALT), formerly known as glutamic-pyruvic transaminase (GPT), EC 2.6.1.2, is widely present in animals, plants, microorganisms, and cultured cells. It catalyzes the transamination reaction between amino acids and keto acids, playing an important role in amino acid metabolism. This kit employs a 2,4-dinitrophenylhydrazine (DNPH) colorimetric method for the determination of ALT activity: ALT catalyzes the transamination of alanine with α-ketoglutarate to generate pyruvate and glutamate. The addition of 2,4-dinitrophenylhydrazine solution to the reaction system both terminates the enzymatic reaction and condenses with pyruvate to form pyruvate-2,4-dinitrophenylhydrazone, which appears brownish-red under alkaline conditions and exhibits a characteristic absorbance peak at 520 nm. By measuring the increase in absorbance at this wavelength, the ALT enzyme activity in the sample can be precisely calculated.
Storage and Shipping
Storage
Store at 2-8°C
Shipped In
Wet ice
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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