α-Amylase from Bacillus amyloliquefaciens, CAS No.9000-85-5

CAS: 9000-85-5 Cat. No.: A299002 EC Number: 232-560-9 PubChem CID: 135337197
AVAILABLE TO ORDER
GRADE & PURITY EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. liquid, ≥250 units/g
Accession #
P00692
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Size
Status
Price
Qty
10ml
A299002-10ml
1
$42.90
50ml
A299002-50ml
3
$148.90
250ml
A299002-250ml
3
$514.90
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Why this grade

EnzymoPure™, liquid, ≥250 units/g EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 12 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. Product  is from Bacillus amyloliquefaciens and is supplied as a liquid. α-Amylase has been used in various plant studies, such as metabolism studies in Arabidopsis. α-Amylase from Bacillus amyloliquefaciens has been used to hydrolyze sweetpotato amylopectin to identify cluster structure. The enzyme has been used to hydrolyze sago palm starch to reducing sugars, which are then used for ethanol fermentation by Saccharomyces cerevisiae. The enzyme catalyzes amylolysis of gelatinised waxy maize starch to produce reducing sugars.

Specifications

Product Name
α-Amylase from Bacillus amyloliquefaciens, CAS No.9000-85-5
Synonyms
1, 4-α-D-Glucan Glucano-hydrolase | EC 3.2.1.1 | BAN 480L
Grade
EnzymoPure™
Specifications & Purity
EnzymoPure™, liquid, ≥250 units/g
Biochemical and Physiological Mechanisms
An endoamylase that randomly hydrolyzes α-(1→4)-glycosidic linkages in amylose and amylopectin. The breakdown products are oligosaccharides and dextrins of varying chain length. This enzyme is active at high temperatures (70?90 °C). α-Amylase is an endoa
Accession #
CAS
9000-85-5
Enzyme Commission Number
3.2.1.1
Molecule Type
Enzyme
Legal Information
BAN is a trademark of Novozymes Corp.
Storage and Shipping
Concentration
liquid, ≥250 units/g
Storage
Store at 2-8°C
Shipped In
Wet ice
Unit definition
One unit is the amount of enzyme which dextrinizes 5.26 g of dry starch per hour under standard conditions.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

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📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

11 results found

Lot NumberCertificate TypeDateItem
D2613002Certificate of AnalysisApr 15, 2026 A299002
K2525064Certificate of AnalysisNov 27, 2025 A299002
B2226509Certificate of AnalysisSep 08, 2025 A299002
E2523083Certificate of AnalysisMay 29, 2025 A299002
G2310708Certificate of AnalysisApr 08, 2025 A299002
G2310713Certificate of AnalysisApr 08, 2025 A299002
G2310714Certificate of AnalysisApr 08, 2025 A299002
G2310722Certificate of AnalysisApr 08, 2025 A299002
E2310095Certificate of AnalysisFeb 07, 2025 A299002
B2226495Certificate of AnalysisFeb 21, 2022 A299002
B2226499Certificate of AnalysisFeb 21, 2022 A299002

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Documents & Articles
Citations of This Product
References
1. Shuo Zou, Yiren Zhang, Qiaojun Wang, Lixue Yang, Emad Karrar, Qingzhe Jin, Hui Zhang, Gangcheng Wu, Xingguo Wang.  (2021)  Effect of palm stearin on the physicochemical characterization and capsaicinoid digestion of Sichuan hotpot oil.  FOOD CHEMISTRY,      [PMID:34649199] [10.1016/j.foodchem.2021.131167]
2. Hui Cao, Denghui Meng, Xutao Liu, Tai Ye, Min Yuan, Jinsong Yu, Xiuxiu Wu, Yan Li, Fengqin Yin, Fei Xu.  (2021)  Extraction of Pb(II) from wheat samples via dual-frequency ultrasound-assisted enzymatic digestion and the mechanisms of its interactions with wheat proteins.  FOOD CHEMISTRY,      [PMID:34116494] [10.1016/j.foodchem.2021.130247]
3. Qu Yaobaixue, Yang Zhao-Guang, Li Haipu, Yao Jie, Qiu Bo.  (2020)  Ultrasound-Assisted Enzymatic Extraction Method for Multi-element Analysis of Rice.  Food Analytical Methods,  13  (8): (1549-1555).  [PMID:] [10.1007/s12161-020-01779-3]
4. Ting Xie, Li Zhou., Laichuang Han, Cuiping You, Zhongmei Liu, Wenjing Cui, Zhongyi Cheng, Junling Guo, Zhemin Zhou.  (2024)  Engineering hyperthermophilic pullulanase to efficiently utilize corn starch for production of maltooligosaccharides and glucose.  FOOD CHEMISTRY,      [PMID:38402758] [10.1016/j.foodchem.2024.138652]
5. Bing Zheng, Tianxiang Ao, Xiaole Zhao, Yi Chen, Jianhua Xie, Xingcai Gao, Li Liu, Xiaobo Hu, Qiang Yu.  (2024)  Comprehensive assessment of the anti-obesity effects of highland barley total, insoluble, and soluble dietary fiber through multi-omics analysis.  FOOD RESEARCH INTERNATIONAL,      [PMID:38876588] [10.1016/j.foodres.2024.114535]
6. Bing Zheng, Xiaole Zhao, Tianxiang Ao, Jianhua Xie, Yi Chen, Yueyao Gui, Xingcai Gao, Xiaobo Hu, Qiang Yu.  (2024)  Exploring and comparing the anti-obesity mechanisms of defatted rice bran total dietary fiber, insoluble dietary fiber, and soluble dietary fiber based on multi-omics technologies.  Food Frontiers,  (2): (708-721).  [PMID:] [10.1002/fft2.353]
7. Mengfan Lin, Changrong Wang, Wenfei Wu, Qingsong Miao, Zebin Guo.  (2024)  Quality, improvement of soluble dietary fiber from Dictyophora indusiata by-products by steam explosion and cellulase modification: Structural and functional analysis.  Food Chemistry-X,      [PMID:39791116] [10.1016/j.fochx.2024.102084]
8. Bing Zheng, Xiaole Zhao, Tianxiang Ao, Yi Chen, Jianhua Xie, Xingcai Gao, Li Liu, Xiaobo Hu, Qiang Yu.  (2024)  The role of bound polyphenols in the anti-obesity effects of defatted rice bran insoluble dietary fiber: An insight from multi-omics.  FOOD CHEMISTRY,      [PMID:38986204] [10.1016/j.foodchem.2024.140345]
9. Mengmeng Zhang, Nana Yang, Caiyun Wu, Hexin Zhang, Chengxin Wang, Ting Zhang, Hongjie Lei.  (2025)  Physical modifications of dietary fibers from kiwifruit pomace: Physicochemical, structural and functional properties.  FOOD CHEMISTRY,      [PMID:40267669] [10.1016/j.foodchem.2025.144422]
10. Weifan Gao, Yan Cui, Mingwei Xue, Xintao Liu, Xin Meng, Wutong Jiang, Xinyu Jiang, Dali Zhang, Mingzhu Zheng, Jingsheng Liu.  (2025)  Study on the ultrasonic treatment of black rice dietary fiber and its effect on anthocyanin adsorption.  ULTRASONICS SONOCHEMISTRY,      [PMID:40451112] [10.1016/j.ultsonch.2025.107409]
11. Chun-Min Ma, Xiao-Han Li, Xiao-Peng Wang, Chun-Li Song, Xin-Huai Zhao.  (2025)  Impact of the Enzyme-Hydrolyzed Pumpkin (Cucurbita moschata Duch.) Pulp on the Chemical and Textural Features of Cake Batter and Chiffon Cake.  ACS Food Science & Technology,      [PMID:] [10.1021/acsfoodscitech.5c00335]
12. Jing Liang, Jiayan Xie, Yue Gu, Jianhua Xie, Yi Chen, Bing Zheng, Yue Qiu, Xiaobo Hu, Qiang Yu.  (2025)  Optimization and identification of bound polyphenols from rice bean (Vigna umbellata) skin dietary fiber, antioxidant activities and α-glucosidase inhibition mechanism.  Food Chemistry-X,      [PMID:41323664] [10.1016/j.fochx.2025.103278]
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