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Why this grade
for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Storage & shipping
Store at -80°C,Avoid repeated freezing and thawing Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Product Info
Included Materials:
A 2-part assay buffer and dilution buffer are included.
Description
Topo IV is a type II topoisomerase that is encoded by two genes parC and parE in E. coli. It is essential for decatenation and chromosomal segregation in E. coli; thus, topo IV tends to prefer intermolecular strand passing (catenating and decatenating reactions) over relaxation reactions. Topoisomerase IV is also the target for quinolone-based antibacterial agents which act by subverting the enzyme into a DNA damaging agent. Aladdin offers purified Topo IV for use in all aspects of drug development and screening assays. The enzyme is very active in decatenating kDNA (TG2013) but will also relax supercoiled plasmid DNA. It is less robust in relaxation activity, however. Topo IV is purified according to Peng and Marians.
Shipping&storage
The enzyme is shipped on dry ice and should be stored at -70°C. We also recommend that the enzyme be aliquoted after the first thaw. Note that repeated rounds of freeze/thaw will lead to activity loss.
Quality Control Tests
Topo IV was overexpressed and purified using the published methods. A single band on SDS-PAGE was detected by CB staining for each subunit. Cross contamination by topo I was assessed by assaying for relaxation of supercoiled DNA under conditions optimized for type I activity. Under these conditions, after 2 hours of incubation with pBR322, no relaxation products were detectable.
Assay Conditions
Reactions contained the following (20 ul): 40 mM Tris-HCl (pH 7.5), 6 mM MgCl2, 10 mM DDT, 100 mM potassium glutamate, 40 uM ATP, 50 ug BSA/ml, 0.2 ug kDNA. Reactions were terminated after 30 min at 37°C with SDS (1%) followed by proteinase K digestion (50 ug/ml, 15 min at 56°C). Reaction products were run on a 1% agarose gel containing 0.5 ug/ml ethidium bromide (monitored with hand held UV light to resolve decatenation products), destained with distilled water (20 min room temperature) and photographed.
Specifications
Product Name
E. coli Topoisomerase IV
Molecule Type
Small molecule
Storage and Shipping
Storage
Store at -80°C,Avoid repeated freezing and thawing
Shipped In
Dry ice packs + Cold packs
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →✅ Certificate of Analysis (COA)
Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →🔬 Specification Sheet
Full quality attributes and acceptance criteria for this grade.
View spec sheet →Advanced Data
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Documents & Articles
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