GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Pre-adsorbed?Pre-adsorbed — cross-reactive antibodies removed by adsorption for higher specificity. Use in multiplex/IHC to cut background and cross-reaction.1.0 mg/mL
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C. Avoid freeze/thaw cycle. Protect from light.
Images
Goat Anti-Rabbit IgG H&L (AF488) (Ab223312) - IF/ICC IF analysis of HA tag (Green) in HEK293T cells transfected with a mCherry-HA tag. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant HA tag Antibody (Ab106771) at 1.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab223312) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
Goat Anti-Rabbit IgG H&L (AF488) (Ab223312) - Flow Cytometry Flow Cytometry analysis of HA tag expression by HEK293T cells transfected with a mCherry-HA tag (red) compared to an isotype control (blue). The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes and then stained with Recombinant HA tag Antibody (Ab106771) at 0.5 μg/mL for 30 minutes at 4°C in the dark. Then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab223312) at a dilution of 1/500 for 1 hour at room temperature in the dark. Blue - Rabbit IgG (Ab170215).
Application
Application
Dilution info
Flow Cytometry
1/100-1/1000
IF/ICC
1/100-1/1000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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