Lectin from Ulex europaeus (gorse, furze) - peroxidase conjugate, lyophilized powder

Cat. No.: L755071

GRADE & PURITY peroxidase conjugate, lyophilized powder

Storage

Store at 2-8°C

Shipped In

Wet ice

Overview

General Western Blot Protocol:

• Glycoprotein sample size: 500ng
• Lectin Concentration: 0.1ug/ml

1. Load samples at 500 ng of glycoprotein per lane
2. Run 4-20% Bis-Tris SDS page gel
3. Transfer gel to a PVDF membrane
4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
7. Detect using chemiluminescent substrate (CPS1-120)

Specifications

Specifications & Purity

peroxidase conjugate, lyophilized powder

Biochemical and Physiological Mechanisms

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N, N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170, 000 daltons. Late

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.