Licoricidin (LCD) is isolated from Glycyrrhiza uralensis Fisch , possesses anti-cancer activities. Licoricidin (LCD) inhibit SW480 cells ( IC 50 =7.2 μM) by inducing cycle arrest, apoptosis and autophagy , and is a potential chemopreventive or chemotherapeutic agent against colorectal cancer. Licoricidin (LCD) inhibits Lung Metastasis by inhibition of tumor angiogenesis and lymphangiogenesis as well as changes in the local microenvironment of tumor tissues the anticarcinogenic effect. Licoricidin enhanced gemcitabine-induced cytotoxicity in Osteosarcoma (OS) cells by inactivation of the Akt and NF-κB pathways in vitro and in vivo . Licoricidin blocks UVA-induced photoaging via ROS scavenging, limits the activity of MMP-1 , it can be considered as an active ingredient in new topically applied anti-ageing formulations.
In Vitro
Licoricidin (LCD) (0-20 μM; 24 hours) dose-dependently inhibits the viability of colon cancer cell lines with various pathological and genetic characters, namely SW480, HCT116, SW620 and LoVo cells, with IC 50 values of 7.2, 5.4, 4.5 and 5.1 μM, respectively. Licoricidin (LCD) (0-20 μM; 0-12 hours) induces cell apoptosis was accompanied with the activation of caspase-3 by cleavage in a time- and dose-dependent manner. Licoricidin (LCD) (0-20 μM; 0-12 hours) induces autophagy of SW480 cells, increases the cleavage of LC3-I to LC3-II and the degradation of p62 in a time and dose dependent manner. Licoricidin (LCD) (0-5 μg/ml; 18 hours) inhibits cell migration, MMP-9 secretion, and VCAM expression in 4T1 cells. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Cell Viability AssayCell Line: SW480, HCT116, SW620 and LoVo cells Concentration: 0-20 μM Incubation Time: 24 hours Result: Decreased colon cancer cell lines viability. Western Blot AnalysisCell Line: SW480 cells Concentration: 0 μM, 2.5 μM, 5 μM, 10 μM, 20 μM Incubation Time: 0 hours, 1 hour, 3 hours, 6 hours, 12 hours Result: Induced cell apoptosis.
In Vivo
Licoricidin (LCD) (intraperitoneal injection; 5, 10, or 20 mg/kg; once daily; 15 days) significantly inhibited the growth of SW480 xenografts in nude mice with an inhibitory rate of 43.5% . Licoricidin (LCD) (intraperitoneal injection; 5, 10, or 20 mg/kg; once daily; 32 days) reduces pulmonary metastasis and the expression of CD45, CD31, HIF-1α, iNOS, COX-2, and VEGF-A in tumor tissues, additionally, decreases protein expression of VEGF-R2, VEGF-C, VEGF-R3, and LYVE-1 in tumor tissues of licoricidin-treated mice. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: SW480 xenografted tumor growth in nude mice Dosage: 5, 10, or 20 mg/kg Administration: Intraperitoneal injection; once daily; 15 days Result: Decreased tumor volumes. Animal Model: BALB/c mouse orthotopic modelDosage: 5, 10, or 20 mg/kg Administration: Intraperitoneal injection; 5, 10, or 20 mg/kg; once daily; 32 days Result: Inhibited Lung Metastasis of 4T1 Murine Mammary Carcinoma cells.
Form:Solid
