GRADE & PURITY BioReagent, Native, ≥95%(SDS-PAGE), from Human plasma; Pre-lyophilization Protein Concentration: See COA

Accession #

P03952

Bioactivity

See COA

Overview

Purified from fresh frozen human plasma. Human Prekallikrein is a single chain gamma globulin glycoprotein that participates in the early phase of contact activation, kinin formation and fibrinolysis. Prekallikrein purity is determined by SDS-PAGE and shows no reduction upon incubation with 2-mercaptoethanol. Activity is determined via clotting assay.

Buffer composition = 4 mM Sodium Acetate-HCl/0.15 M NaCl/pH 5.3

Extinction Coefficient (1%) =11.7

Molecular Weight = 86,000 daltons

The above protein was purified from Human plasma units that test negative for antibodies to HBc, HCV, HIV 1/2, HTLV-I/II, and non reactive for HBsAg, HIV-1 RNA, HCV RNA, HBV DNA and Syphilis by FDA approved tests. Donors are screened for CJD.

Specifications

Product Name

Prekallikrein (PK), CAS No.9055-02-1

Grade

BioReagent, Native

Specifications & Purity

BioReagent, Native, ≥95%(SDS-PAGE), from Human plasma; Pre-lyophilization Protein Concentration: See COA

Biochemical and Physiological Mechanisms

Participates in the surface-dependent activation of blood coagulation. Activates, in a reciprocal reaction, coagulation factor XII/F12 after binding to negatively charged surfaces. Releases bradykinin from HMW kininogen and may also play a role in the ren

Bioactivity

See COA

Accession #

P03952

Predicted molecular weight

86 kDa

CAS

9055-02-1

Molecule Type

Protein

Storage and Shipping

Concentration

from Human plasma; Pre-lyophilization Protein Concentration: See COA

Reconstitution

We suggest hydrating the protein with a good quality water to the original volume (See COA). After hydration aliquot into a useful (one time use) size and freeze at -80℃.

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ long term. Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.