GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.Endotoxin Free?Endotoxin-free — verified below sensitive endotoxin limits. Use in cell culture, in-vivo, and immunology work where endotoxin triggers responses.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)1.0 mg/mL
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
📋
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
📚
Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Recombinant DYKDDDDK Antibody, clone P16A17, could be used for WB, Flow Cytometry, IF/ICC, IP, ELISA and so on.
Specifications
Product Name
Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody - Primary antibody, specific to DYKDDDDK epitope tag
Synonyms
DDDDK epitope tag antibody | DDDK antibody | ddk antibody | DYKDDDDK (also known as the FLAG® tag sequence) antibody | DYKDDDDK antibody | DYKDDDDK epitope tag antibody | DYKDDDDK tag antibody | ECS epitope tag antibody | ECS tag antibody | Enterokinase C
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
DYKDDDDK tag
Host species
Mouse
Specificity
DYKDDDDK epitope tag
Immunogen
Synthetic DYKDDDDK peptide
Positive Control
WB: DYKDDDDK tag transfected HEK293, whole cell lysate Flow Cytometry: HEK293 cells transfected with Flag tag IF/ICC: HEK293 cells transfected with Flag and mCherry tags, HEK293 cells transfected with Flag and eGFP tags IP: HEK293 cells transfected with F
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab224136) - Western Blot All lanes: Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab224136) at 0.5 μg/mL Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 115 kDa Observed band size: 64, 119 kDa Exposure time: 30.0 s
Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab224136) - Flow Cytometry Flow Cytometry analysis of HEK293-EGFP-Flag tag cells labelling DYKDDDDK tag with Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab224136) (Right panel) compared with Mouse IgG (Ab170221) - Isotype Control (left panel) and detected with Goat Anti-Mouse IgG H&L (AF647) (Ready to use) (Ab218689).
Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab224136) - IF IF analysis of DYKDDDDK tag (red) in HEK293-EGFP-Flag Tag cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant DYKDDDDK tag (Binds to Flag tag) Antibody (Ab224136) at 2.0 μg/mL in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF647) (Ready to use) (Ab218689) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue), and the EGFP protein is green. Images were taken on the confocal laser scanning microscope.
Application
Application
Dilution info
IP
1/200
IF/ICC
1/200
WB
1/1000 - 1/20000
Flow Cytometry
1/200 – 1/20000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
We use cookies to ensure the website functions properly and, where permitted, to improve your experience. You can manage your preferences at any time in Settings. Learn more in our Cookie Policy.
Shall we send you a message when we have discounts available?
Remind me later
Thank you! Please check your email inbox to confirm.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
ZJ25F0623100
