UltraBio™ 2×Taq Master Mix (Dye Plus) - BioReagent, DNase, RNase free, PCR Reagent, Suitable for molecular biology, for DNA and RNA applications, 2×

Cat. No.: FP1509090

GRADE & PURITY Suitable for molecular biology, BioReagent, DNase, RNase free, PCR Reagent, for DNA and RNA applications, 2×

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

PCR

Overview

UltraBio™ 2×Taq Master Mix (Dye Plus) is specifically designed for qualitative PCR. It contains a loading dye (bromophenol blue) and a gel electrophoresis buffer, allowing direct loading of the PCR reaction onto an agarose gel without additional steps. This ready-to-use mix includes hot-start Taq DNA Polymerase, dNTPs, and optimized buffer with magnesium. Users only need to add template and primers to perform amplification, and the products can be directly analyzed by agarose gel electrophoresis. This product ensures high amplification efficiency and specificity while simplifying operation and saving time.

Intended Use: Qualitative PCR requiring post-amplification analysis.

Instructions for Use

1. Preparation of Reaction Mix

a. Thaw all reagents at room temperature or on ice and mix gently before use.

b. Prepare a 50 µL reaction system as follows:

Component	Volume	Final Concentration
2×Taq Master Mix (Dye Plus)	25 μL	1×
※ Primers	—	—
※ Sample	—	—
ddH₂O	To 50 μL	—
Total Volume	50 μL	—

※ Add primers and sample according to project requirements.

c. It is recommended to aliquot the reaction mix to avoid repeated freeze-thaw cycles.

d. Prepare the reaction mix on ice or a cooling rack.

2. Thermal Cycling Protocol           

Note

Because this product contains bromophenol blue dye for agarose gel electrophoresis, the solution appears bluish‑purple. Mix thoroughly by vortexing before use.

Specifications

Specifications & Purity

BioReagent, DNase, RNase free, PCR Reagent, Suitable for molecular biology, for DNA and RNA applications, 2×

Stability And Storage

Store at -20℃ long term (12 months). Avoid freeze/thaw cycle. Upon receipt, it is recommended to aliquot.

Storage

Store at -20°C, Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, DNase, RNase free, for DNA and RNA applications, PCR Reagent, Suitable for molecular biology