Storage buffer: 10mM Tris HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6
Product Introduction
This product is composed of 9 linear double stranded DNA fragments, stored in a 1xLoading Buffer, with dense bands that require high concentration gel and low voltage electrophoresis. It is suitable for accurately confirming the size of DNA fragments. The size range is 50-500bp, which are 50bp, 100bp, 150bp, 200bp, 250bp, 300bp, 350bp, 400bp, and 500bp respectively; 250 bp is the bright band, with a concentration 2.5 times that of other bands; In the 5 ul product, each strip contains about 40 ng, and the highlight strip contains about 100 ng.
Usage suggestions
1. It is recommended to sample 5 ul and increase the sample size appropriately for wide adhesive holes.
2. It is recommended to use 2.5-3.5% Agarose or 5% PAGE gel, voltage 4-10 v/cm, and 0.5xTBE buffer for electrophoresis. Pay attention to replacing the electrophoresis buffer in time and using the newly prepared gel to achieve ideal results.
3. Stain with EB or other nucleic acid dyes, and observe the electrophoretic bands under UV light.
matters needing attention
1. This product has been saved in a 1xLoading Buffer and can be directly used for electrophoresis. It is easy to use and the electrophoresis image is clear.
2. When using agarose gel containing EB for electrophoretic detection, the bromophenol blue band can be electrophoretic to about 2/3 of the distance, otherwise the small segment will become dark because EB is separated from DNA.
3. The accompanying 5xLoading buffer can be used for sample detection.
5x Loading Buffer components:
10 mM Tris-HCl，5 mM EDTA， pH7.6， 0.03% bromophenol blue, 0.03% xylene blue, 30% glycerol
Product component
| D745352 | Component | 100 T | 500T | Storage | | D745352A | DNA Ladder (50-500bp) | 500 μL
| 5× 500 μL | -20℃. Avoid freeze/thaw cycle. | | D745352B | 5xLoading buffer | 1 mL
| 5× 1 mL | -20℃. Avoid freeze/thaw cycle. |
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