GRADE & PURITYBioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.His Tag?His tag grade — recombinant protein bearing a His tag for affinity purification/detection. Use to purify, immobilize, or detect the tagged protein.≥90%(SDS-PAGE)≥100 U/mL, Protein concentration: see COA
Bioactive, ActiBioPure™, High Performance, EnzymoPure™, His Tag, ≥90%(SDS-PAGE), ≥100 U/mL, Protein concentration: see COA ActiBioPure™,Bioactive,High Performance,His Tag,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Endo F2 is a highly specific recombinant endoglycosidase derived from Elizabethkingia meningoseptica. It cleaves core-fucosylated biantennary complex-type and high-mannose oligosaccharides linked to asparagine residues from glycoproteins and glycopeptides. Endo F2 digests biantennary complex glycans approximately 20 times faster than high-mannose glycans. Its enzymatic activity is unaffected by the presence or absence of core fucosylation. Nevertheless, Endo F2 exhibits no activity against hybrid-type, triantennary or tetraantennary glycans.
Components List
E755020
Component
400U
1KU
Storage
E755020A
Endo-β-N-acetylglucosaminidase F2
400U
1KU
-20℃. Avoid freeze/thaw cycle.
E755020B
10×GlycoBuffer 3 (500 mM Sodium acetate pH4.5)
1000μl
1000μl
-20℃. Avoid freeze/thaw cycle.
Operating Protocol
1. Before use, take out the Endo F2 reagent and centrifuge at 10,000 rpm for 10 seconds to collect all liquid at the bottom of the tube.
2. Mix 10 µg bovine fibrinogen with 1 µL of 500 mM Sodium Acetate (pH 4.5), add Endo F2, and top up with purified water to a total reaction volume of 20 µL. Mix gently, then incubate the reaction mixture at 37°C for 1 hour.
3. Analyze the digested products via SDS-PAGE or mass spectrometry.
Remark: Incubation duration and enzyme dosage should be optimized according to practical experimental conditions.
Precautions
Wear a lab coat and disposable nitrile gloves during operation to safeguard personal safety and health. This product is for research use only.
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ long term (18 months). Avoid freeze/thaw cycle.
Unit definition
Quantity of enzyme sufficient to eliminate >95% carbohydrates from 10 μg denatured bovine fibrinogen in 10 μL reaction mix upon incubation at 37 °C for 1 h.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
