What solution should be used to dissolve Proteinase K when used? What is the common working concentration? At what degree does it need to be stored?
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What solution should be used to dissolve Proteinase K when used? What is the common working concentration? At what degree does it need to be stored?
·Reviewed·Published ·Updated ·8 min read
What solution should be used to dissolve Proteinase K when used? What is the common working concentration? At what degree does it need to be stored?
The effective pH range of proteinase K is pH4.0~12.5, and the optimal pH range is pH7.5-8.0. Please try to use the buffer solution with the above appropriate pH according to the experimental requirements, such as: 10mM Tris-HCl pH7.4-7.8, for the preparation of proteinase K solution. The commonly used working concentration of proteinase K is 10~100ug/ml, and the concentration of storage solution is usually 100~200mg/ml, stored at -20℃, and should be used as soon as possible after diluted to the working concentration, avoiding prolonged storage.
Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
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Aladdin Scientific. "What solution should be used to dissolve Proteinase K when used? What is the common working concentration? At what degree does it need to be stored?" Aladdin Knowledge Base, updated Aug 28, 2024. https://www.aladdinsci.com/us_en/faqs/what-solution-should-be-used-to-dissolve-proteinasek-en.html
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