EnzymoPure™, ≥25u/mg,derived from Arthrobacter EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 27 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate.
Application：
This enzyme is used for the enzymatic determination of triglyceride in serum by couplingwith glycerophosphate dehydrogenase (G-3-PDH) and L-a-glycerophosphate oxidase (GPO)or pyruvate kinase and lactate dehydrogenase in clinical diagnosis.
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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| 1. Pengyun Zhao, Lingxin Meng, Jingmei Xu, Jinxuan Lei, Kuirong Feng, Tian Lan, Zhe Wang. (2023) Poly(arylene ether ketone sulfone)s functionalized with adenosine triphosphate-modified graphene oxide as proton exchange membranes for fuel cells. EUROPEAN POLYMER JOURNAL, [PMID:] [10.1016/j.eurpolymj.2023.112605] |
| 2. Yuqin Wang, Pingping Fan, Shanyu Zhang, Liying Wang, Xinyue Li, Wendong Jia, Yao Liu, Kefan Wang, Xiaoyu Du, Panke Zhang, Shuo Huang. (2022) Discrimination of Ribonucleoside Mono-, Di-, and Triphosphates Using an Engineered Nanopore. ACS Nano, [PMID:36475606] [10.1021/acsnano.2c09662] |
| 3. Baoxia Liu, Yaoyao Zhang, Yuanqiang Hao, Xu Zhu, Yintang Zhang, Yanli Zhou, Hongliang Tan, Maotian Xu. (2022) All-in-One Luminescent Lanthanide Coordination Polymer Nanoprobe for Facile Detection of Protein Kinase Activity. ANALYTICAL CHEMISTRY, [PMID:35853147] [10.1021/acs.analchem.2c01307] |
| 4. Yue Teng, Guangqing Song, Rong Chen, Xiao Zhang, Yongjun Sun, Haixia Wu, Biming Liu, Yanhua Xu. (2021) Carboxymethyl β-cyclodextrin immobilized on hydrated lanthanum oxide for simultaneous adsorption of nitrate and phosphate. Journal of the Taiwan Institute of Chemical Engineers, [PMID:] [10.1016/j.jtice.2021.11.020] |
| 5. Jia Xu, Habib Khan, Li Yang. (2021) Hydrogel Paper-Based Analytical Devices: Separation-Free In Situ Assay of Small-Molecule Targets in Whole Blood. ANALYTICAL CHEMISTRY, [PMID:34709797] [10.1021/acs.analchem.1c03347] |
| 6. Yang Li, Zhenzhen Cheng, Chunlei Zhao, Cong Gao, Wei Song, Liming Liu, Xiulai Chen. (2021) Reprogramming Escherichia coli Metabolism for Bioplastics Synthesis from Waste Cooking Oil. ACS Synthetic Biology, [PMID:34337931] [10.1021/acssynbio.1c00155] |
| 7. Lihua Liu, Linlin Zhao, Dandan Cheng, Xinyi Yao, Yan Lu. (2019) Highly Selective Fluorescence Sensing and Imaging of ATP Using a Boronic Acid Groups-Bearing Polythiophene Derivate. Polymers, 11 (7): (1139). [PMID:31277286] [10.3390/polym11071139] |
| 8. Guimei Wang, Xiaoxue Jiang, Nanyan Fu. (2019) Near-infrared squaraine fluorescent probe for imaging adenosine 5′-triphosphate in live cells. DYES AND PIGMENTS, [PMID:] [10.1016/j.dyepig.2019.107698] |
| 9. Kai Song, Yejing Wang, Yu Li, Chaoxiang Ding, Rui Cai, Gang Tao, Ping Zhao, Qingyou Xia, Huawei He. (2019) A Convenient, Rapid, Sensitive, and Reliable Spectrophotometric Assay for Adenylate Kinase Activity. MOLECULES, 24 (4): (663). [PMID:30781833] [10.3390/molecules24040663] |
| 10. Lei Tian, Hongsu Zhao, Zuzhi Zhao, Jiali Zhai, Zipin Zhang. (2019) A facile voltammetric method for detection of heparin in plasma based on the polyethylenimine modified electrode. Analytical Methods, 11 (10): (1324-1330). [PMID:] [10.1039/C8AY02722F] |
| 11. Lijun Geng, Xudong Yu, Yajuan Li, Yanqiu Wang, Yongquan Wu, Jujie Ren, Fengfeng Xue, Tao Yi. (2019) Instant hydrogel formation of terpyridine-based complexes triggered by DNA via non-covalent interaction. Nanoscale, 11 (9): (4044-4052). [PMID:30768104] [10.1039/C8NR08532C] |
| 12. Renxuan Liu, Tao Sha, Qiuhong Zhou, Bei Nie. (2018) Copper-surrogated galvanic displacement of silver dendrite imprinted on flexible and transparent silk fibroin membrane as a SERS-active substrate and sub-dividable catalyst. APPLIED SURFACE SCIENCE, [PMID:] [10.1016/j.apsusc.2018.11.223] |
| 13. Pang Jiawei, Lu Yuexiang, Gao Xinyu, Song Panshu, Yang Fengyi, Liu Yueying. (2018) On-off-on luminescent pyrophosphate probe based on the use of Mn-doped ZnS quantum dots and using Eu(III) as a mediator. MICROCHIMICA ACTA, 185 (10): (1-8). [PMID:30259119] [10.1007/s00604-018-3003-3] |
| 14. Cui Liu, Qiang Zhang, Nianqi An, Jing Wang, Linlin Zhao, Yan Lu. (2018) A new water-soluble polythiophene derivative as a probe for real-time monitoring adenosine 5′-triphosphatase activity in lysosome of living cells. TALANTA, [PMID:29501170] [10.1016/j.talanta.2018.02.008] |
| 15. Fangfang Li, Xuan Hu, Fengyi Wang, Baozhan Zheng, Juan Du, Dan Xiao. (2017) A fluorescent “on-off-on” probe for sensitive detection of ATP based on ATP displacing DNA from nanoceria. TALANTA, [PMID:29310233] [10.1016/j.talanta.2017.09.091] |
| 16. Jinying Sun, Yang Liu, Simin Lv, Zhirong Huang, Liao Cui, Tie Wu. (2015) An Electrochemical Sensor Based on Nitrogen-doped Carbon Nanofiber for Bisphenol A Determination. ELECTROANALYSIS, 28 (3): (439-444). [PMID:] [10.1002/elan.201500287] |
| 17. Qingkun Kong, Meng Li, Chao Ma, Hongmei Yang, Shenguang Ge, Mei Yan, Jinghua Yu. (2015) Ultrasensitive electrochemiluminescence aptasensor based on a graphene/polyaniline composite film modified electrode and CdS quantum dot coated platinum nanostructured networks as labels. RSC Advances, 5 (86): (70345-70351). [PMID:] [10.1039/C5RA12674F] |
| 18. Xiaohua Zhu, Tingbi Zhao, Zhou Nie, Yang Liu, Shouzhuo Yao. (2015) Non-Redox Modulated Fluorescence Strategy for Sensitive and Selective Ascorbic Acid Detection with Highly Photoluminescent Nitrogen-Doped Carbon Nanoparticles via Solid-State Synthesis. ANALYTICAL CHEMISTRY, [PMID:26202861] [10.1021/acs.analchem.5b02167] |
| 19. He Yunfei, Liao Lifu, Xu Canhui, Wu Rurong, Li Shijun, Yang Yanyan. (2014) Determination of ATP by resonance light scattering using a binuclear uranyl complex and aptamer modified gold nanoparticles as optical probes. MICROCHIMICA ACTA, 182 (1): (419-426). [PMID:] [10.1007/s00604-014-1350-2] |
| 20. Yurong Tang, Yu Zhang, Yingying Su, Yi Lv. (2013) Highly sensitive resonance light scattering bioassay for heparin based on polyethyleneimine-capped Ag nanoclusters. TALANTA, [PMID:24054670] [10.1016/j.talanta.2013.06.028] |
| 21. Jian-Hong Wu, Xiao-Shui Li, Yong Zhao, Weiping Zhang, Lin Guo, Yu-Qi Feng. (2011) Application of liquid phase deposited titania nanoparticles on silica spheres to phosphopeptide enrichment and high performance liquid chromatography packings. JOURNAL OF CHROMATOGRAPHY A, [PMID:21470615] [10.1016/j.chroma.2011.03.019] |
| 22. Wenbo Li, Xin Yan, Wenli Xia, Linguo Zhao, Jianjun Pei. (2024) Development of an efficient dimethylallyl diphosphate regeneration system by a co-immobilization of multi-enzyme cascade for the one-pot synthesis of prenylated flavonoids. BIOCHEMICAL ENGINEERING JOURNAL, [PMID:] [10.1016/j.bej.2024.109584] |
| 23. Pei Jin Xie, Ming Li Ye, Zhong Yang Hu, Guang Wen Pan, Yan Zhu, Jia Jie Zhang. (2011) Determination of levels of adenosine phosphates in blood by ion chromatography. CHINESE CHEMICAL LETTERS, [PMID:] [10.1016/j.cclet.2011.09.008] |
| 24. Yuanyuan Su, Minghui Gu, Cheng Li, Dequan Zhang, Yuqing Ren, Li Chen, Shaobo Li, Xiaochun Zheng. (2024) Development of a rhodamine-based fluorescent probe for ATP detection for potential applications in meat freshness assessment. FOOD CHEMISTRY, [PMID:38615529] [10.1016/j.foodchem.2024.139209] |
| 25. Qinnan Yang, Fei Kong, Ziyi Liu, Anxing Jian, Yue Wang, Yong He, Xiaoli Li, Bingquan Chu. (2025) Fe3+-assisted nitrogen-doped carbon dot fluorescence switch for ultrasensitive detection of acetyl-CoA in live microalgal cells. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, [PMID:41344011] [10.1016/j.saa.2025.127281] |
| 26. Yuwei Liu, Yuting Wang, Ziyi Liu, Chang Li, Qiang Yu, Jianhua Xie, You Long, Qianzhu E, Yi Chen. (2025) Flavor modulation of braised He Bao carp (Cyprinus carpio) by natural plant spices: Insights from GC–MS, GC–IMS, E-nose, E-tongue, and molecular docking analyses. Food Bioscience, [PMID:] [10.1016/j.fbio.2025.108101] |
| 27. Jinnian Wang, Haoming Zhao, Shijun Tong, Xingjian Zhang, Shuyu Ding, Han Zhang, Zhong-Hua Yang. (2026) Cascade biocatalysis for pyridoxal 5′-phosphate synthesis with ATP autonomy via polyphosphate kinase. BIOORGANIC CHEMISTRY, [PMID:41564697] [10.1016/j.bioorg.2026.109518] |
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F2428418