K2202734Determine the necessary mass, volume, or concentration for preparing a solution.
Horseradish Peroxidase Maleimide Conjugate (0.5mg×3), CAS No.H405810
AVAILABLE TO ORDER
Accession #
P00432
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Why this grade
for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Storage & shipping
Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Description：
HRP Maleimide Conjugate is designed to facilitate the conjugation of the HRP to proteins, peptides and ligands that contain a free sulfhydryl (-SH) groups. Activated HRP contains 1–3 maleimide groups for each HRP molecule.
Product Information：
Volume/Quantity : 0.5 mg x 3 vial
Conjugation : Peroxidase from Horseradish (HRP) was conjugated to Sulfo-SMCC (Product M123456).
Product Form : Horseradish Peroxidase Maleimide Conjugate. Lyophilized in 10 mM sodium phosphate, 0.115 mM NaCl, 40 mM sucrose, pH 6.6.
Preservative : None
Protocol：
Firstly return the vial to room temperature.
Reconstitute the contents of the vial with 100μL of buffer or water to obtain a 5 mg/mL solution of HRP Maleimide.
Note: Do not vortex or heat the HRP Maleimide.
Dissolve the sulfhydryl compound to be conjugated at 2-5 mg/ml in the buffer.
Note: We recommend that the thiol-containing molecule is dissolved in the pH range 6.5-7.5 buffer. 0.1M sodium phosphate, 0.15M NaCl, 10mM EDTA at pH 7.2 is commonly employed. Maleimides react with sulfhydryls at pH 6.5-7.5 to form stable thioether bonds. EDTA may help to prevent metal catalysed oxidation of thiols. Use of a 4:1 (HRP Maleimide:antibody) molar ratio in the conjugation reaction usually results in high-activity conjugates. This vial contains 0.5 mg of HRP Maleimide Conjugate and can effectively modify 0.5 mg of immunoglobulin.
Immediately mix the thiol-containing molecule and HRP Maleimide solutions and allow them to react for 2 hours at room temperature. To increase HRP incorporation, extend reaction time up to 12 hours.
If desired, desalt, dialyze or purify the conjugate.
Storage：
Store at −20 °C to −80 °C. Reconstitute this reagent immediately before use. Do not prepare stock solutions for storage. Discard any unused reconstituted reagent.
Specifications
Product Name
Horseradish Peroxidase Maleimide Conjugate (0.5mg×3), CAS No.H405810
Synonyms
HRP Maleimide (0.5mg×3) | Horseradish Peroxidase Maleimide Activated (0.5mg×3)为：HRP Maleimide (0.5mg×3) | Horseradish Peroxidase Maleimide Activated (0.5mg×3)
Accession #
CAS
H405810
Molecule Type
Enzyme
Storage and Shipping
Storage
Store at -20°C
Shipped In
Ice chest + Ice pads
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →✅ Certificate of Analysis (COA)
Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →🔬 Specification Sheet
Full quality attributes and acceptance criteria for this grade.
View spec sheet →Advanced Data
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Solution Calculators
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