Monensin Solution (1,000X) - sterile-filtered,BioReagent,1000X

Cat. No.: M1456520

GRADE & PURITY Sterile-filtered, BioReagent, 1000X

Synonyms

Monensin Sodium

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Flow Cytometry

Overview

  Monensin is a protein transport inhibitor commonly used to enhance intracellular cytokine staining signals by blocking transport processes during cell activation.       Especially useful for the intracellular staining of cytokines, monensin leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum . Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest.

  This monensin is in the sodium salt form, which is supplied as a 1000X working solution in 70% Ethanol.

Usage Protocol

  Dilute the 1000X solution to 1X in the cell culture medium. 

Precautions

  It is recommended that cells are cultured with monensin for ≤ 24 hours, as this can become toxic for cell viability.

Specifications

Synonyms

Monensin Sodium

Specifications & Purity

sterile-filtered, BioReagent, 1000X

Stability And Storage

Store at -20℃ long term (12 months) or store at 4℃ short term (6 months).

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, Sterile-filtered

Application

• Application: Dilution info
• Flow Cytometry:

  1/1000;