Recombinant c-Kit Antibody (AF647) - Primary antibody, specific to Mouse c-Kit

Cat. No.: Ab213865

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

P80D41

Conjugation

AF647

Overview

Recombinant c-Kit Antibody, clone P80D41, could be used for Flow Cytometry.

Specifications

Product Name

Recombinant c-Kit Antibody (AF647) - Primary antibody, specific to Mouse c-Kit

Synonyms

C Kit antibody | c-Kit antibody | c-Kit Ligand antibody | CD117 antibody | Kit antibody | Kit Ligand antibody | KIT oncogene antibody | KIT proto oncogene receptor tyrosine kinase antibody | KIT_HUMAN antibody | Mast cell growth factor receptor antibody |

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration

Host species

Rat

Specificity

Mouse c-Kit

Immunogen

Mouse bone marrow mast cells

Positive Control

Flow Cytometry: Mouse bone marrow cells

Conjugation

AF647

Excitation (nm)

Red Laser(633nm)

Emission(nm)

668nm

Ex/Em(nm)

Ex:650nm;Em:668nm

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein A purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: Recombinant c-Kit Antibody (AF647) (Ab213865)

Recombinant c-Kit Antibody (AF647) (Ab213865) - Flow Cytometry
Two-color flow cytometric analysis of c-Kit expression on C57BL/6 mouse bone marrow cells. C57BL/6 mouse bone marrow cells were stained with CD45R Rat mAb (FITC) (Ab191667) and either Rat IgG (AF647) (Ab190071; Left panel) or Recombinant c-Kit Antibody (AF647) (Ab213865; Right panel) at 0.2μg/test.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

KIT Tclin Mast/stem cell growth factor receptor Kit (0 Activities)

Discover Target: KIT

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)