GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5mg/mL
CD16: Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor which binds to IgG1, IgG2a and IgG2b.
Mediates neutrophil activation by IgG complexes redundantly with Fcgr4.
CD32: Receptor for the Fc region of complexed immuno
Host species
Rat
Specificity
Mouse CD16/CD32
Immunogen
Mouse BALB/c Macrophage J774
Positive Control
Flow Cytometry: C57BL/6 mouse splenocytes
Conjugation
AF594
Excitation (nm)
Yellow-Green Laser(594nm)
Emission(nm)
618nm
Ex/Em(nm)
Ex:590nm;Em:618nm
Grade
ExactAb™, Recombinant, Validated
Light Chain Type
lambda
Purification Method
Protein A purified
Concentration
0.5mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images
Recombinant CD16/CD32 Antibody (AF594) (Ab223917) - Flow Cytometry Flow cytometry analysis of CD16/CD32 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD16/CD32 Antibody (AF594) (Ab223917) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF594) (Ab190241).
Recombinant CD16/CD32 Antibody (AF594) (Ab223917) - Flow Cytometry Flow cytometry analysis of CD16/CD32 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD16/CD32 Antibody (AF594) (Ab223917) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (AF594) (Ab190241). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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