GRADE & PURITYAnimal Free?Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material.Carrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Bioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.≥95%(SDS-PAGE)See COA
Accession #
P00734
Protein Tag
No tag
Expression system
HEK293
Endotoxin Concentration
<0.1 EU/μg
Bioactivity
The activity of Recombinant Human Coagulation Factor II/ Thrombin Protein (rp303098) was measured by the rate of free chromophore formation from Chromozym TH (B1008216) thrombin substrate monitored by absorbance at 405 nm. Activity ranges from 1000-5000 units/mg.
Animal Free,Carrier Free,Bioactive,ActiBioPure™,High Performance,≥95%(SDS-PAGE),See COA ActiBioPure™,Animal Free,Bioactive,Carrier Free,High Performance for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Specifications
Product Name
Recombinant Human Coagulation Factor II/Thrombin Protein
Synonyms
coagulation factor II (thrombin) receptor-like 2 | Coagulation factor II receptor-like 2 (protease-actovated receptor 3) | Coagulation factor II receptor-like 2 | Coagulation Factor II | F2 | PAR-3 | PAR3proteinase-activated receptor 3 | protease-activate
Grade
ActiBioPure™, Animal Free, Bioactive, Carrier Free, High Performance
Specifications & Purity
Animal Free, Carrier Free, Bioactive, ActiBioPure™, High Performance, ≥95%(SDS-PAGE), See COA
Biochemical and Physiological Mechanisms
Coagulation Factor II, commonly known as thrombin, is an essential component of the coagulation cascade in which it converts fibrinogen to fibrin, activates factors V, VII, VIII, XIII and forms complexes with protein C and thrombomodulin. It also activate
Bioactivity
The activity of Recombinant Human Coagulation Factor II/ Thrombin Protein (rp303098) was measured by the rate of free chromophore formation from Chromozym TH (B1008216) thrombin substrate monitored by absorbance at 405 nm. Activity ranges from 1000-5000 units/mg.
31.7 & 5.6 kDa, under reducing conditions; 35.0 &7.0 kDa, under non-reducing conditions.
Molecule Type
Protein
Storage and Shipping
Concentration
See COA
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
Recombinant Human Coagulation Factor II/Thrombin Protein (rp303098) - Enzyme Activity
The activity of Recombinant Human Coagulation Factor II/Thrombin Protein (rp303098) was measured by the rate of free chromophore formation from Chromozym TH thrombin substrate monitored by absorbance at 405 nm. Activity ranges from 1000-5000 units/mg.
Materials:
Buffer : 50mM Tris-HCl, 0.1% BSA and 227 mM NaCl, pH 8.3
Substrate: Chromozym TH (B1008216)
Procedures：
1. Dilute Recombinant Human Coagulation Factor II/Thrombin Protein (rp303098) to 0.28 µg/mL in Assay Buffer.
2. Dilute Chromozym TH (B1008216) to 400 µM in Assay Buffer.
3. Load 50 µL of 0.28 µg/mL Recombinant Human Coagulation Factor II/Thrombin Protein (rp303098) into a plate, and start the reaction by adding 50 µL of 400 µM Substrate. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of Substrate.
4. Read at 405 nm (absorbance) in kinetic mode for 15 minutes.
Recombinant Human Coagulation Factor II/Thrombin Protein (rp303098) - Enzyme
Activity
The activity of Recombinant Human Coagulation Factor II/Thrombin Protein (rp303098) was measured by the rate of free chromophore formation from Chromozym TH thrombin substrate monitored by absorbance at 405 nm. Activity ranges from 1000-5000 units/mg.
Materials:
Buffer : 50mM Tris-HCl, 0.1% BSA and 227 mM NaCl, pH 8.3
Substrate: Chromozym TH (B1008216)
Procedures：
1. Dilute Recombinant Human Coagulation Factor II/Thrombin Protein (rp303098) in Assay Buffer.
2. Dilute Chromozym TH (B1008216) to 400 µM in Assay Buffer.
3. Load 50 µL of Recombinant Human Coagulation Factor II/Thrombin Protein (rp303098) into a plate, and start the reaction by adding 50 µL of 400 µM Substrate. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of Substrate.
4. Read at 405 nm (absorbance) in kinetic mode for 15 minutes.
Recombinant Human Coagulation Factor II/Thrombin Protein (rp303098) - SDS-PAGE
3 μg/lane of Recombinant Human Coagulation Factor II/Thrombin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the bands at 31.7 & 5.6 kDa under reducing conditions and 35.0 & 7.0 kDa under non-reducing conditions.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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