Recombinant Protein A, AF647 conjugate, CAS No.91932-65-9

CAS: 91932-65-9 Cat. No.: rp303258 EC Number: 295-242-9 PubChem CID: 363902062

GRADE & PURITY BioReagent, Azide Free, Suitable for Immunofluorescence(IF), 1.0 mg/mL

Accession #

P02976

Protein Tag

No tag

Expression system

E. coli

Overview

Aladdin offers recombinant protein A conjugated to our LumiDye™ AF Dyes (Table 1).

Table 1. Spectral characteristics of protein A conjugates.

Cat.No.	Conjugate	λmax (nm)	Em (nm
rp303252	FITC	498	517
rp303253	AF488	490	525
rp303254	AF555	553	568
rp303256	AF594	590	618
rp303258	AF647	650	668

Aladdin recombinant protein A is a modification of the protein structure based on natural Staphylococcus aureus protein A. It consists of four Fc binding domain fragments linked together, with a molecular weight of 27KD. Compared with natural protein A, it removes the binding site with the immunoglobulin Fab region, enhances its binding ability with the Fc region, and improves the stability and alkali resistance of the protein. Recombinant protein A can separate and purify antibodies of different subtypes of mammals or genetic engineering recombinant proteins containing Fc fragments of antibodies from ascites, serum, cell culture supernatant or cell extracts.

Recombinant Protein A was conjugated with LumiDye™ AF647 under optimal conditions and subsequently purified to remove unconjugated free dyes. This conjugate can be used for immunoglobulin detection in Flow Cytometry and IF and for assay development. Red Fluorescence. Excitation/Emission wavelength= 650 nm/668 nm.

Specifications

Product Name

Recombinant Protein A, AF647 conjugate, CAS No.91932-65-9

Synonyms

Protein A | Staphylococcal Protein A | Staphylococcal Protein A Recombinant | Immunoglobulin G-binding protein A | gG-binding protein A | Immunoglobulin G binding protein A | SPA | rat-PA | Protein A, AF647 Conjugate | Protein A – AF647

Grade

Azide Free, BioReagent, Suitable for Immunofluorescence(IF)

Specifications & Purity

Suitable for Immunofluorescence(IF), BioReagent, Azide Free, 1.0 mg/mL

Biochemical and Physiological Mechanisms

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immu

Conjugation

AF647

Excitation (nm)

Red Laser(633nm)

Emission(nm)

668nm

Ex/Em(nm)

Accession #

P02976

Note

Note: Because staining protocols vary with application, the appropriate dilution of the protein A conjugates should be determined empirically.

CAS

91932-65-9

Molecule Type

Protein

Storage and Shipping

Concentration

1.0 mg/mL

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at-20°C for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Images

IF/ICC: Recombinant Protein A, AF647 conjugate (rp303258)

Recombinant Protein A, AF647 conjugate (rp303258)  – IF/ICC 

IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Citatuzumab (anti-EpCAM) (Ab209902; Isotype type: Human IgG1) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Recombinant Protein A, AF647 conjugate (rp303258) at 1/500 dilution for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with Hoechst 33342 (B113845) (blue) at 1/1000 dilution for 5 minutes. Images were taken on the confocal laser scanning microscope. .

Flow Cytometry: Recombinant Protein A, AF647 conjugate (rp303258)

Recombinant Protein A, AF647 conjugate (rp303258) - Flow Cytometry

Flow Cytometry analysis of HT-29 cells labelling EpCAM (red) with Citatuzumab (anti-EpCAM) (Ab209902; Isotype type: Human IgG1). Recombinant Protein A, AF647 conjugate (rp303258) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  1/100-1/1000 ;

• IF/ICC:

  1:500 ;

References

1. Li Yangyang, Zhu Zhengwei, Qu Wenli, Yang Qing, Liu Yan, Wang Qiao, Duan Shuo, Wu Jine, Gong Zhiyong, Xu Lin.  (2023)  A highly sensitive immunochromatographic assay for lead ions in drinking water based on antibody-oriented probe and silver enhancement.  EUROPEAN FOOD RESEARCH AND TECHNOLOGY,  249  (12): (3097-3103).  [PMID:] [10.1007/s00217-023-04351-5]

2. Jin Li, Xintong Li, Yulian Wang, Qianxu Zhou, Guoqing Shi.  (2024)  Utilization of Fc-specific peptide to orientally immobilize antibodies markedly improves the analytical performance of lateral flow immunoassay strips for aflatoxin B1.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2024.112404]

3. Shuo Yang, Jinglong Qu, Yang Qin, Shuaibing An, Kaiqiang Huang, Yaling Wei, Jun Xi.  (2026)  Enhanced ultra-sensitive detection of peanut allergen Ara h 3 on immunochromatographic paper based on surface-enhanced Raman spectroscopy and AuNBs@PDA dual signal amplification strategy.  FOOD CHEMISTRY,      [PMID:41638166] [10.1016/j.foodchem.2026.148209]

4. Pan Cheng, Chuang Zhang, Xiupu Lu, Daiqing Peng, Pengju Zhao, Tao Mei, Shunxing Zhang, Qihao Guo, Ke Liu, Dong Wang.  (2026)  Protein A functionalized hierarchically ordered nanofibrous sponge enabling high-efficiency IgG purification.  COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,      [PMID:] [10.1016/j.colsurfa.2026.140254]