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BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Urea, also known as carbamide, is the main nitrogen-containing end product of protein metabolism in mammals and certain fish species, and it is also the nitrogen fertilizer with the highest nitrogen content currently available. Urea detection methods can be broadly categorized into chemical methods and enzymatic methods. The latter is considered an indirect method, where urea is first broken down into ammonium ions by urease, and then the amount of ammonium ions generated is detected based on the Berthelot reaction. The detection principle of this kit is based on the hydrolysis of urea by urease, producing ammonia and carbon dioxide. Under alkaline conditions, ammonia reacts with phenol and other reagents to form blue indophenol. The amount of indophenol formed is proportional to the urea content. By measuring the absorbance at 560 nm, this kit can be used to determine the urea content (formerly referred to as blood urea nitrogen, BUN) in samples such as human and animal plasma, serum, and urine. However, urine samples are best pretreated before detection. This product is intended for research purposes only and is not suitable for clinical diagnosis or other applications.
Comprehensive hazard, handling, storage, and regulatory compliance document.
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