β-Glucosidase, CAS No.9001-22-3

CAS: 9001-22-3 Cat. No.: G755171 EC Number: 232-589-7

GRADE & PURITY Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, ≥10U/mg powder; 10-60 U/mg protein

Bioactivity

≥10U/mg powder; 10-60 U/mg protein

Overview

Beta-glucosidase is a glucosidase enzyme that acts upon β1->4 bonds linking twoglucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). It is oneof the cellulases, enzymes involved in the decomposition of cellulose and relatedpolysaccharides; more specifically, an exocellulase with specificity for a variety ofbeta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducingresidues in beta-D-glucosides with release of glucose.

Applications

This enzyme is useful for structural investigations of carbohydrates and for theenzymatic determination of α-amylase when coupled with α-glucosidase in clinicalanalysis.

Product Information

Source: Sweet almond

Appearance: Light yellow amorphous powder, lyophilized

Form: Freeze dried powder

Molecular Weight:approx. 110 kDa

Activity: 10U/mg-solid or more (containing approx. 50% of BSA)

Contaminants: α-Amylase < 5.0×10⁻⁴%

Isoelectric point: 7.3

pH Stability: pH 6.0-9.0 (25°C, 64hr)

Optimum pH: 5.5

Thermal stability: below 50°C (pH 7.3, 1hr)

Optimum temperature: 50-55°C

Michaelis Constant: 2.8×10⁻³M (p-Nitrophenyl-ß-D-glucopyranoside), 3.3×10⁻³M (2,4-Dichlorophenyl-ß-D-glucopyranoside)

Structure: 2 subunits per mol of enzyme

Stabilizers: Bovine serum albumin (BSA), glutathione (reduced)
Stability: Stable at-20°C for at least 6 months (A decrease in activity of ca. 10% may occur at5°C within 6 months)

Specifications

Product Name

β-Glucosidase, CAS No.9001-22-3

Synonyms

beta-glucosidase | amygdalase | beta-D-glucoside glucohydrolase | cellobiase | gentobiase | β-D-Glucoside glucohydrolase | b-D-Glucosidase | b-D-Glucoside glycohydrolase | EC 3.2.1.21 | gentiobiase | emulsin | elaterase | aryl-beta-glucosidase | beta-D-gl

Grade

ActiBioPure™, Bioactive, High Performance, Native, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, ≥10U/mg powder; 10-60 U/mg protein

Bioactivity

≥10U/mg powder; 10-60 U/mg protein

Predicted molecular weight

~110 kDa

CAS

9001-22-3

Molecule Type

Enzyme

Product Line

biological_products

Storage and Shipping

Concentration

≥10U/mg powder; 10-60 U/mg protein

Reconstitution

Reconstitute in ddH2O.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (2 years). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

The amount of enzyme which catalyzes the formation of one micromole of p-Nitrophenol per minute at 37°C.

Frequently Asked Questions

What is ActiBioPure??

ActiBioPure? is one of Aladdin Scientific's premium product lines, formulated for high-purity applications across multiple workflows. Compared to standard grades in the same workflow, ActiBioPure? products typically offer tighter specifications, more rigorous QC, and stronger lot-to-lot consistency.

What does Bioactive mean?

Bioactive indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 9001-22-3.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.