Enterokinase human

Cat. No.: E755265

GRADE & PURITY Recombinant, ≥90%(SDS-PAGE), expressed in CHO cells

Overview

The enzyme enterokinase is a serine protease that is encoded by a 3696 nucleotide cDNA that contains an open reading frame of 3057 nucleotides. The encoded precursor is cleaved to form an active protein that contains a 784 amino acid heavy chain followed by a 235 amino acid light chain that are linked by one or more disulfide bonds. The mRNA is found to be expressed only in small intestine and the protein is found in enterocytes of duodenum and proximal jejunum . The human enterokinase gene is localized to chromosome 21q21.

Application:

Enterokinase has been used in the activation of pancreatic enzyme preparation. It has been used for the activation and determination of trypsin activity in pancreatic enzyme secretion.

Specifications

Product Name

Enterokinase human

Grade

Recombinant

Specifications & Purity

Recombinant, ≥90%(SDS-PAGE), expressed in CHO cells

Biochemical and Physiological Mechanisms

The enterokinase enzyme is a glycoprotein that catalyzes the conversion of trypsinogen to trypsin, which activates the zymogens pancreatic digestive enzymes. Deficiency of this enzyme causes protein deficiency as zymogens do not get activated. Congenital

Molecule Type

Enzyme

Storage and Shipping

Concentration

expressed in CHO cells

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Frequently Asked Questions

What is the purity of this product, and how is it determined?

This product is supplied at ≥90% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.