ER-Tracker Red

Cat. No.: E745770

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

Precautions：

ER-Tracker Red (1mM) will solidify on ice or at lower temperatures such as 4℃ and stick to the bottom, walls or lid of the centrifuge tube. It should be thawed completely in a water bath at 20-25℃ prior to use. For trace amounts of liquid, centrifuge briefly before each use to collect the liquid at the bottom of the tube.Fluorescence intensity decays. Avoid light to minimize the quenching of fluorescence.Coverslips and slides are required but not supplied. They can be ordered from .The pharmacological activity of glibenclamide may affect some functions of the ER. Variable expression of sulfonylurea receptors in some specific cells may result in non-ER-specific staining.ER-Tracker Red is suitable for staining ER in live cells, but not ER in fixed cells. When cells stained with ER-Tracker Red need to be fixed, fix cells with 4% paraformaldehyde at 37℃ for 2 minutes.ER-Tracker Red stained cells cannot be permeabilized with Triton X-100 which will result in the loss of ER-Tracker Red fluorescent staining.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1.Preparation of ER-Tracker Red working solutiona.Dilute an appropriate amount of ER-Tracker Red (1mM) in the ER-Tracker Red Dilution Buffer at a ratio of 1:1000. For example, mix 1µl of ER-Tracker Red with 1ml of ER-Tracker Red Dilution buffer to obtain the ER-Tracker Red working solution.b.Prewarm the ER-Tracker Red working solution at 37℃ before use.Note: The dilution ratio of ER-Tracker Red can be adjusted within the range of 1:1000-1:3000 based on the staining results. To minimize the staining background, a lower concentration of ER-Tracker Red is preferred as long as an acceptable staining result can be obtained.2.Fluorescent labeling of ERa.Remove the cell culture medium and wash cells grown on coverslips with an appropriate amount of solution such as Hanks' Balanced Salt Solution with Ca2+ & Mg2+ (, C0219). For staining of suspension cells, centrifuge to collect cells and refer to the staining method for adherent cells .b.Remove the wash buffer, add the ER-Tracker Red working solution pre-incubated at 37℃, and incubate at 37℃ for 15-30 min.c. Remove the ER-Tracker Red working solution and wash cells 1-2 times with cell culture medium.d.Examine cells by fluorescence microscope or confocal microscope.e.If the ER-Tracker Red stained cells need to be fixed, fix with 4% paraformaldehyde for 2 min at 37℃. After fixation, wash cells 2-3 times with appropriate wash buffer for 5 minutes each, and then counterstain cells or mount cells with an appropriate antifade mounting medium. Note: ER-Tracker Red stained cells cannot be permeabilized with Triton X-100 which will result in the loss of ER-Tracker Red fluorescent staining.

Specifications

Stability And Storage

Stored at -20 ℃, effective for six months. ER Tracker Red needs to be stored at -20 ℃ away from light.

Storage

Protected from light, Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.