QUALITÄT & REINHEITCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.1.0 mg/mL
Recombinant protein corresponding to Human beta-2-microglobulin
Positive Kontrolle
WB: HeLa, Raji, THP-1 and HepG2 Whole cell Lysates ICC/IF: HeLa cells
Konjugation
Unconjugated
Note
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Isotyp
IgG2a
Lichterketten-Typ
kappa
SDS-PAGE
150.0 KDa
Verfahren zur Reinigung
Protein A purified
Konzentration
1.0 mg/mL
Product Line
Biologische Produkte
Lagerung und Versand
Form
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Bilder
beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 13.7 kDa Observed band size: 13.8 kDa Exposure time: 43.0 s
beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 13.7 kDa Observed band size: 13.8 kDa Exposure time: 43.0 s
beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 13.7 kDa Observed band size: 13.8 kDa Exposure time: 43.0 s
beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 13.7 kDa Observed band size: 13.8 kDa Exposure time: 43.0 s
beta 2 Microglobulin Mouse mAb (Ab169199) - ICC/IF IF analysis of beta 2 Microglobulin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta 2 Microglobulin Mouse mAb (Ab169199) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
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What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (beta 2 Microglobulin Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
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