beta Actin Mouse mAb (TRITC) - Primärer Antikörper, specific to beta actin, IgG1

Cat. No.: Ab176323

GRADE & PURITY ExactAb™, Azide Free, Validated, Ex:557nm, Em:576nm, 0.5 mg/mL

Application

IF/ICC

Host species

Maus

Reactive species

Maus, Mensch, Rat

Isotype

Mouse IgG1

Clone Number

FG01/2D10

Conjugation

TRITC

Übersicht

Application:
IF: 1:1000

Specifications

Product Name

beta Actin Mouse mAb (TRITC) - Primärer Antikörper, specific to beta actin, IgG1

Synonyme

A26C1A antibody | A26C1B antibody | ACTB antibody | ACTB_HUMAN antibody | Actin beta antibody | Actin cytoplasmic 1 antibody | Actin, cytoplasmic 1, N-terminally processed antibody | Actx antibody | b actin antibody | b-actin antibody | Beta cytoskeletal

Spezifikationen & Reinheit

ExactAb™, Validated, Azide Free, Ex:557nm, Em:576nm, 0.5 mg/mL

Wirtsspezies

Maus

Spezifität

beta actin

Immunogen

Synthetic peptide in beta Actin

Positive Kontrolle

IF: Hela

Konjugation

TRITC

Anregung (nm)

Green Laser(532nm)

Emission (nm)

576nm

Ex/Em(nm)

Ex:557nm;Em:576nm

Note

Azide Free, Em:576nm, Ex:557nm, ExactAb™, Validated

Isotyp

IgG1

Lichterketten-Typ

kappa

Verfahren zur Reinigung

Protein A purified

Konzentration

0.5 mg/mL

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Bilder

IF: beta Actin Mouse mAb (TRITC) (Ab176323)

beta Actin Mouse mAb (TRITC) (Ab176323) - IF
IF analysis of beta Actin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Mouse mAb (TRITC) (Ab176323) at 1/1000 dilution in blocking buffer overnight at 4°C. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Anwendung

Anwendung

Dilution info

IF/ICC

1:1000

Zugehörige Ziele (menschlich)

ACTB Tbio Actin, cytoplasmic 1 (0 Activities)

Discover Target: ACTB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Azide Free mean?

Azide Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C, protected from light. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (beta Actin Mouse mAb (TRITC)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.