c-Fos Mouse mAb - Primärer Antikörper, specific to c-Fos, Mouse IgG1

Cat. No.: Ab096159

QUALITÄT & REINHEIT ExactAb™, Validated, 1.0 mg/mL

Application

WB

Host species

Maus

Reactive species

Affe, Mensch, Rat

Isotype

Mouse IgG1

Clone Number

7D6-A9-D4

Conjugation

Unconjugated

Übersicht

Mouse anti Human c-Fos Antibody, Monoclonal (7D6-A9-D4), could be used for WB and so on.

Application:

WB: 1/500-1/1000

Protein Function

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.

Spezifikationen

Product Name

c-Fos Mouse mAb - Primärer Antikörper, specific to c-Fos, Mouse IgG1

Synonyme

Cellular oncogene fos | G0/G1 switch regulatory protein 7

Spezifikationen & Reinheit

ExactAb™, Validated, 1.0 mg/mL

Wirtsspezies

Maus

Spezifität

c-Fos

Immunogen

Purified recombinant human FOS protein fragments expressed in E.coli (AA 1-380).

Positive Kontrolle

WB: HeLa (serum starved 36 hours, then treated with 20% FBS for 2 hours) cell lysates.

Konjugation

Unconjugated

Note

ExactAb™, Validated

Isotyp

Mouse IgG1

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

1.0 mg/mL

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: c-Fos Mouse mAb (Ab096159)

c-Fos Mouse mAb (Ab096159) - Western Blot
All lanes: c-Fos Mouse mAb (Ab096159) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 67 kDa
Exposure time: 91.4 s

Anwendung

Anwendung

Dilution info

WB

1/500-1/1000

Zugehörige Ziele (menschlich)

FOS Tbio Proto-oncogene c-Fos (0 Activities)

Discover Target: FOS

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (c-Fos Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.