GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.≥98%(HPLC)
BioReagent,≥98%(HPLC) BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Desiccated Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Fura-2 is a specific fluorescent indicator for intracellular calcium ions (Ca²⁺), and it is one of the most commonly used fluorescent probes for detecting intracellular calcium ion concentration.Fura-2 AM is a cell-permeable fluorescent dye. It exhibits weak fluorescence, with a maximum excitation wavelength of 369 nm and a maximum emission wavelength of 478 nm. Notably, its fluorescence intensity does not change with variations in calcium ion concentration. After entering cells, Fura-2 AM can be cleaved by intracellular esterases to form Fura-2, which is then trapped inside the cells. When Fura-2 binds to calcium ions, its maximum excitation wavelength is 335 nm (the maximum excitation wavelength varies with ion concentration), and the maximum emission wavelength is 505 nm. For practical detection, the recommended excitation wavelength is 340 nm and the emission wavelength is 510 nm. For dual-wavelength detection, the recommended excitation wavelengths are 340 nm and 380 nm.
For intracellular calcium ion detection, the commonly used concentration of Fura-2 AM is 0.5–5 μM. Typically, probe loading can be achieved by incubating cells with an appropriate solution containing 0.5–5 μM Fura-2 AM at 4–37 °C for 15–60 minutes.
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