CD154/CD40L Mouse mAb - Primärer Antikörper, specific to TRAP/CD40L, Mouse IgG2a

Cat. No.: Ab132316

GRADE & PURITY Carrier Free, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, Flow Cytometry, Functional Assay, IP

Host species

Maus

Reactive species

Affe, Mensch

Isotype

Mouse IgG2a

Clone Number

5C8

Conjugation

Unconjugated

Übersicht

Mouse anti Human/Monkey CD154/CD40L Antibody, Monoclonal (5C8), could be used for in vitro blocking of CD40/CD40L signaling, in vivo blocking of CD40/CD40L signaling, IP, Flow and so on.

Application:
in vitro blocking of CD40/CD40L signaling: Use at an assay dependent concentration.

in vivo blocking of CD40/CD40L signaling: Use at an assay dependent concentration.

IP: Use at an assay dependent concentration.

Flow: Use at an assay dependent concentration.
Protein Function
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.

Specifications

Product Name

CD154/CD40L Mouse mAb - Primärer Antikörper, specific to TRAP/CD40L, Mouse IgG2a

Synonyme

IGM | IMD3 | CD154 antigen | CD154 | CD40 antigen ligand | CD40 Ligand | CD40L | CD40-L | CD40LG | CD40LIGM | gp39 | hCD40L | HIGM1 | T-B cell-activating molecule | T-BAM | T-cell antigen Gp39 | TNF-related activation protein | TNFSF5 | TNFSF5IMD3 | TRAP

Spezifikationen & Reinheit

Carrier Free,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

Wirtsspezies

Maus

Spezifität

TRAP/CD40L

Immunogen

Human D1.1 T cell line

Konjugation

Unconjugated

Note

Azide Free, Carrier Free, PBS Only, Validated

Isotyp

Mouse IgG2a

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

See COA

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at 2-8°C,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Bilder

ELISA: CD154/CD40L Mouse mAb (Ab132316)

CD154/CD40L Mouse mAb (Ab132316) - ELISA
Immobilized Recombinant Human CD40 Ligand/TNFSF5 protein (rp189423) at 1.0 μg/mL can bind CD154/CD40L Mouse mAb (Ab132316) with the EC₅₀ of 3.301 ng/mL.

Anwendung

Anwendung

Dilution info

IP

Use at an assay dependent concentration.

Flow Cytometry

Use at an assay dependent concentration.

in vitro blocking of CD40/CD40L signaling

Use at an assay dependent concentration

in vivo blocking of CD40/CD40L signaling

Use at an assay dependent concentration

Zugehörige Ziele (menschlich)

CD40LG Tbio CD40 ligand (0 Activities)

Discover Target: CD40LG

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Azide Free mean?

Azide Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Do not freeze. Freezing may cause irreversible damage to this material.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD154/CD40L Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.