CD28 Mouse mAb (PE) - Primärer Antikörper, specific to CD28, Mouse IgG1

Cat. No.: Ab185380

QUALITÄT & REINHEIT ExactAb™, Validated, Ex:565nm, Em:575nm, 5 μL/test

Application

Flow Cytometry

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG1

Clone Number

CD28.2

Conjugation

PE

Übersicht

Application:
Flow: 5 μL/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Spezifikationen

Product Name

CD28 Mouse mAb (PE) - Primärer Antikörper, specific to CD28, Mouse IgG1

Synonyme

CD28 antigen (Tp44) | CD28 antigen | CD28 molecule | CD28 | MGC138290 | T-cell-specific surface glycoprotein CD28 | Tp44 | CD 28 antibody | CD28 antibody | CD28 antigen antibody | CD28 molecule antibody | CD28_HUMAN antibody | MGC138290 antibody | T cell

Spezifikationen & Reinheit

ExactAb™, Validated, Ex:565nm, Em:575nm, 5 μL/test

Wirtsspezies

Maus

Spezifität

CD28

Immunogen

Human CD28 transfected cell line

Positive Kontrolle

Flow: Jurkat

Konjugation

PE

Anregung (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emission (nm)

575nm

Ex/Em(nm)

Ex:565nm;Em:575nm

Note

Em:575nm, Ex:565nm, ExactAb™, Validated

Isotyp

Mouse IgG1

Lichterketten-Typ

kappa

Verfahren zur Reinigung

Protein G purified

Konzentration

5 μL/test

Product Line

Biologische Produkte

Lagerung und Versand

Storage

Store at 2-8°C,Protected from light,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Bilder

Flow Cytometry: CD28 Mouse mAb (PE) (Ab185380)

CD28 Mouse mAb (PE) (Ab185380) - Flow Cytometry
Flow cytometry analysis of CD28 expression by Jurkat cells (red) compared to an isotype control (blue). Cells were stained with CD28 Mouse mAb (PE) (Ab185380) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (PE) (Ab180308). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

Anwendung

Dilution info

Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Zugehörige Ziele (menschlich)

CD28 Tbio T-cell-specific surface glycoprotein CD28 (0 Activities)

Discover Target: CD28

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD28 Mouse mAb (PE)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.